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NEB/Monarch? PCR & DNA Cleanup Kit (5 μg)/T1030S/50 preps

價(jià)格: ￥1188.00 市場(chǎng)價(jià): 1980.00

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貨號(hào): T1030S-50preps

品牌: NEB

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詳情
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常見問題

Description:

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TheMonarchPCR&DNACleanupKitrapidlyandreliablypurifiesupto5μgofconcentrated,high-qualityDNAfromPCRandotherenzymaticreactions.Thekitutilizesabind/wash/eluteworkflowwithminimalincubationandspintimes.Thecolumnsensurezerobufferretentionandnocarryoverofcontaminants,enablingelutionofsampleinvolumesaslowas6μl.Thebuffersprovidedhavebeenoptimized,anddonotrequiremonitoringofpH.ElutedDNAisreadyforuseinrestrictiondigests,DNAsequencing,ligationandotherenzymaticmanipulations.DesignedwithsustainABIlityinmind,MonarchkitsusesignificantlylessplasticandresponsIBLy-sourced,recyclablepackaging.TheprotocolcanalsobemodifiedtoenablethepurificationofsmallerDNAfragments,includingoligonucleotides.

Viewourvideosonprotocols,tips,andrecyclingMonarch.

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APPLICATIONS
PCRcleanup	DNAfromPCRreactionscanbepurifiedafteramplificationtoremovepolymerases,primers,detergents,dNTPs,etc.
Enzymaticreactioncleanup	Modifyingenzymessuchasligases,kinases,nucleases,phosphatasesareefficientlyremovedallowingefficientdesaltingandconcentrationoftheDNAsample.
CDNAcleanup	DNA/RNAcomplexescanbepurifiedpost-reversetranscription/amplificationtoenableremovaloftheRTandpolymeraseaswellasnucleotides.
Labelingcleanup	UnincorporatedrADIolabeledorfluorescentlylabelednucleotidescanberemovedfromtheDNAsubstrate
Plasmidcleanup	Plasmidprepsfromunknownsourcesmaycontaininhibitorsandunwantedcontaminants.Purificationandconcentrationcanbeeasilyachievedusingthiskit.
Oligonucleotidecleanup	ssDNAoligonucleotides(≥18nt)anddsDNAfragments(≥?15bp)canbepurifiedusingtheOligonucleotideCleanupProtocol.

Monarch columns are designed for performance. Monarch columns are designed without a frit, which eliminates buffer retention and the risk of carryover contamination, providing fast, worry-free DNA purification. — Monarchcolumnsaredesignedwithoutafrit,whicheliminatesbufferretentionandtheriskofcarryovercontamination,providingfast,worry-freeDNApurification.

?Advantages:

Eluteinaslittleas6μl
Preventbufferretentionandsaltcarry-overwithoptimizedcolumndesign
Savetimewithfast,user-friendlyprotocols
NoneedtomonitorpH
Buffersandcolumnsavailableseparately
Significantlylessplasticusedwhencomparedwithotherkits?
Responsibly-sourcedandrecyclablepackaging?

Specifications

DNASampleType:	DNAfromPCRandotherenzymaticreactions(e.g.,restrictiondigests,kinasereactions,ligations).*
BindingCapacity:	upto5μg
DNASizeRange:	~50bpto25kb**
TypicalRecovery:	DNA(50bpto10kb):70–90%/?DNA(11–23kb):50–70%
ElutionVolume:	≥6μl
Purity:	A_260/280>1.8andA_260/230>1.8
ProtocolTime:	5minutesofspinandincubationtime
CompatibleDownstream Applications:	ligation,restrictiondigestion,labelingandotherenzymatic manipulations,libraryconstructionandDNAsequencing.

*ssDNAordsDNAoligonucleotidesfromenzymaticreactionscanalsobepurifiedusingtheOligonucleotideCleanupProtocol.
**DNA≥15bpto25kb(dsDNA)andDNA≥18ntto10kb(ssDNA)canalsobepurifiedusingtheOligonucleotideCleanupProtocol.
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Monarch PCR & DNA Cleanup Kit (5 μg) Protocol

Monarch PCR & DNA Cleanup Kit (5 μg) performs equivalently to the leading supplier. Preps were performed according to recommended protocols. 1 μg of a 3 kb DNA fragment was incubated with 1 μM primers and OneTaq? Quick-Load? 2X Master Mix (NEB #M0486). DNA was eluted in 20 μl (NEB) and 40 μl (Qiagen) Elution Buffer. Half of the total elution volume was digested with 5 units of DraIII-HF? (NEB #R3510). The digest and the unused portion of the elution were resolved on a 1% w/v agarose gel along with a representative sample of the starting material. — Prepswereperformedaccordingtorecommendedprotocols.1μgofa3kbDNAfragmentwasincubatedwith1μMprimersandOneTaq?Quick-Load?2XMasterMix(**NEB**#M0486).DNAwaselutedin20μl(NEB)and40μl(**Qiagen**)ElutionBuffer.Halfofthetotalelutionvolumewasdigestedwith5unitsofDraIII-HF?(NEB#R3510).Thedigestandtheunusedportionoftheelutionwereresolvedona1%w/vagarosegelalongwitharepresentativesampleofthestartingmaterial.?

Monarch PCR & DNA Cleanup Kit (5 μg) removes low molecular weight primers from dsDNA samples. Three independent amplicons (267 bp, 520 bp, 1003 bp) were spiked with two oligonucleotides (16-mer, 24-mer) to a final concentration of 1 μM. Half of each mix was purified with the Monarch PCR & DNA Cleanup Kit (5 μg) following the included protocol. Equivalent fractions of the original mixture and the eluted material were resolved on a 20% TBE acrylamide gel at 100V for one hour and stained with SYBR Green II. — Threeindependentamplicons(267bp,520bp,1003bp)werespikedwithtwooligonucleotides(16-mer,24-mer)toafinalconcentrationof1μM.HalfofeachmixwaspurifiedwiththeMonarchPCR&DNACleanupKit(5μg)followingtheincludedprotocol.Equivalentfractionsoftheoriginalmixtureandtheelutedmaterialwereresolvedona20%TBEacrylamidegelat100VforonehourandstainedwithSYBRGreenII.

LearnMoreAboutMonarch

NEBMonarch.com
Optimizeyourresultswithouruniquecolumndesign
EnhanceyourDNApurificationexperience
FeelgoodaboutchoosingMonarch
ChooseMonarchkitsforpurevalue

KitComponents

Thefollowingreagentsaresuppliedwiththisproduct:

	Storeat(°C)	Concentration
Monarch®DNACleanupBindingBuffer	25	1X
Monarch®DNAWashBuffer	25	5X
Monarch®DNAElutionBuffer	25	1X
Monarch®DNACleanupColumns(5μg)	25

Notes:

Thekitshouldbestoredatroomtemperature.Alwayskeepbufferbottlestightlyclosedandkeepcolumnssealedintheenclosedzip-lockbag.Forinformationregardingthecompositionofbuffers,pleaseconsulttheSafetyDataSheets.Properlaboratorysafetypracticesshouldbeemployed,includingtheuseoflabcoats,glovesandeyeprotection.

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NEB/Monarch? PCR & DNA Cleanup Kit (5 μg)/T1030S/50 preps

Description:

KitComponents

Notes:

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