產(chǎn)品概述:儲(chǔ)存條件4℃密封避光保存,短期可放置于室溫。有效期見外包裝。
組分 規(guī)格 | S6009S(10mL) | S6009M(100mL) | S6009L(500mL) |
A液 | 5 mL | 50 mL | 250 mL |
B液 | 5 mL | 50 mL | 250 mL |
產(chǎn)品介紹Super ECL Plus 免疫印跡底物是增強(qiáng)型化學(xué)發(fā)光(ECL)過氧化物酶的底物,可幫助用戶在免疫印跡分析過程中實(shí)現(xiàn)低表達(dá)或高價(jià)值蛋白的檢測(cè)。Super ECL Plus 底物可為使用辣根過氧化物酶(HRP)偶聯(lián)物的免疫印跡實(shí)驗(yàn)提供明亮的信號(hào),對(duì)低表達(dá)或高價(jià)值蛋白檢測(cè)靈敏度很高。該 ECL 底物能夠兼容各種膜、封閉液和寬范圍抗體稀釋液,以出色性能、通用性和高性價(jià)比,滿足用戶的免疫印跡應(yīng)用需求。Super ECL Plus(超敏)特點(diǎn):1. 具有極高靈敏度:檢測(cè)硝化纖維素膜或 PVDF 膜上低表達(dá)或高價(jià)值的蛋白條帶;2. 長(zhǎng)信號(hào)持續(xù)時(shí)間:條件優(yōu)化的情況下,經(jīng)底物孵育的印跡條帶能夠持續(xù)輸出 6 至 8 h 的可檢測(cè)光信號(hào);3. 價(jià)格經(jīng)濟(jì):配方經(jīng)過優(yōu)化,適用于濃度極低的抗體檢測(cè)。? 0.2-1 μg/mL 一抗(或 1 mg/mL 的稀釋 1:1,000-1:5,000)? 10-50 ng/mL 二抗(或 1 mg/mL 的稀釋 1:20,000-1:100,000)注意事項(xiàng)1. 發(fā)光液曝露于強(qiáng)光下時(shí)間過久靈敏度可能有降低,操作時(shí)注意避光。戴手套可以避免在膜上留下手印。2. 長(zhǎng)時(shí)間曝光會(huì)加深背景;蛋白過量,會(huì)使條帶強(qiáng)弱變化失去線性關(guān)系;曝光不足,則條帶模糊或較淺。3. 如果曝光后條帶不佳,可用洗膜緩沖液洗膜,重新孵育二抗,然后重新用 ECL 曝光。4. 使用肉眼可見的預(yù)染色蛋白 Marker 和熒光-放射自顯影曝光標(biāo)簽可精確確定膠片上條帶的位置和大小。5. NaN3 會(huì)抑制 HRP 活性,回收二抗應(yīng)避免使用 NaN3,如必需使用勿超過 0.01%。
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