Nt.AlwI is a nicking endonuclease that cleaves only one strand of DNA on a double-stranded DNA substrate. It is an engineered derivative of AlwI which catalyzes a single strand break 4 bases beyond the 3´ end of the recognition sequence on the top strand.
Product Source
AnE. colistrain containing a chimeric gene encoding the DNA recognition domain of AlwI and the cleavage/dimerization domain of Nt.BstNBI.
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