Rabbit polyclonal antibody raised against full length recombinant CCL2.
Immunogen:
Recombinant protein corresponding to 76 amino acids of bovine CCL2.
Host:
Rabbit
Reactivity:
Bovine
Form:
Lyophilized
Recommend Usage:
ELISA (1:4500)Western Blot (1 ug/mL)The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction:
Store at 4°C on dry atmosphere.After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Publication Reference
1.
Short communication: Localization and expression of monocyte chemoattractant protein-1 in different subcutaneous and visceral adipose tissues of early-lactating dairy cows.Haussler S, Sacre C, Friedauer K, Danicke S, Sauerwein H.J Dairy Sci. 2015 Jun 24. [Epub ahead of print]
2.
CCL2 (Monocyte Chemoattractant Protein-1) in cancer bone metastases.Craig MJ, Loberg RD.Cancer Metastasis Rev. 2006 Dec;25(4):611-9.
3.
Cooperative expression of monocyte chemoattractant protein 1 within the bovine corpus luteum: evidence of immune cell-endothelial cell interactions in a coculture system.Liptak AR, Sullivan BT, Henkes LE, Wijayagunawardane MP, Miyamoto A, Davis JS, Rueda BR, Townson DH.Biol Reprod. 2005 May;72(5):1169-76. Epub 2005 Jan 19.
4.
Human recombinant monocyte chemotactic protein and other C-C chemokines bind and induce directional migration of dendritic cells in vitro.Xu LL, Warren MK, Rose WL, Gong W, Wang JM.J Leukoc Biol. 1996 Sep;60(3):365-71.
Applications
Western Blot (Recombinant protein)
Western blot using CCL2 polyclonal antibody (Cat # PAB16914) shows detection of recombinant bovine CCL2 at 8.8 KDa (arrow) raised in yeast. Protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1 : 10), and probed with CCL2 polyclonal antibody. After washing, membrane was probed with Dylight™ 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody.
Enzyme-linked Immunoabsorbent Assay
Application Image
Western Blot (Recombinant protein)
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Enzyme-linked Immunoabsorbent Assay
Gene Information
Entrez GeneID:
281043
Gene Name:
CCL2
Gene Alias:
SCYA2
Gene Description:
chemokine (C-C motif) ligand 2
Gene Ontology:
Hyperlink
Other Designations:
monocyte chemoattractant protein 1,small inducible cytokine A2 (monocyte chemotactic protein 1)
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