Rabbit polyclonal antibody raised against full length recombinant SARS-CoV nonstructural protein 8 (nsp8).
Immunogen:
Recombinant protein corresponding to full length SARS-CoV nsp8.
Host:
Rabbit
Reactivity:
SARS-CoV
Specificity:
This antibody is directed against SARS-Coronavirus nsp8 protein.
Form:
Liquid
Purity:
The product is neat antiserum.
Recommend Usage:
Immunoelectron Microscopy (1:100)Immunofluorescence (1:300)Immunoprecipitation (1:60)Western Blot (1:1000)The optimal working dilution should be determined by the end user.
Storage Buffer:
In antiserum (0.01% sodium azide).
Storage Instruction:
Store at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage.Aliquot to avoid repeated freezing and thawing.Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
Download
Publication Reference
1.
Ultrastructure and origin of membrane vesicles associated with the severe acute respiratory syndrome coronavirus replication complex.Snijder EJ, van der Meer Y, Zevenhoven-Dobbe J, Onderwater JJ, van der Meulen J, Koerten HK, Mommaas AM.J Virol. 2006 Jun;80(12):5927-40.
2.
Identification and characterization of severe acute respiratory syndrome coronavirus replicase proteins.Prentice E, McAuliffe J, Lu X, Subbarao K, Denison MR.J Virol. 2004 Sep;78(18):9977-86.
3.
Unique and conserved features of genome and proteome of SARS-coronavirus, an early split-off from the coronavirus group 2 lineage.Snijder EJ, Bredenbeek PJ, Dobbe JC, Thiel V, Ziebuhr J, Poon LL, Guan Y, Rozanov M, Spaan WJ, Gorbalenya AE.J Mol Biol. 2003 Aug 29;331(5):991-1004.
Applications
Western Blot (Recombinant protein)
Immunoprecipitation followed by western blotting using SARS-CoV nsp8 polyclonal antibody (Cat # PAB11372) shows a predominant band at 21.8 KDa corresponding to full length SARS protein.Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.
Immunofluorescence
Immunofluorescence Microscopy using SARS-CoV nsp8 polyclonal antibody (Cat # PAB11372) 6 hrs post infection of Vero-E6 cells.Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.