The activity is determined by the ability of MCP-1 to chemoattract THP-1 cells and is detectable starting at 10 ng/mL.
Quality Control Testing:
1 ug/lane in 4-20% Tris-Glycine gel stained with Coomassie BlueLane 1: non-reducing conditionsLane 2: reducing conditions
Storage Buffer:
No additive
Storage Instruction:
Store at -20°C on dry atmosphere.After reconstitution with sterilized water, store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
Result of activity analysis
Human THP-1 cells were allowed to migrate to rat Ccl2 at (0, 0.1, 1, 10, 100 and 1000 ng/mL). After 45 minutes, cells that migrated were counted using a luminescent substrate and displayed on the bar graph above.
Datasheet:
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Applications
Functional Study
SDS-PAGE
Application Image
Functional Study
SDS-PAGE
Gene Information
Entrez GeneID:
24770
Protein Accession#:
P14844
Gene Name:
Ccl2
Gene Alias:
MCP-1,Scya2,Sigje
Gene Description:
chemokine (C-C motif) ligand 2
Gene Ontology:
Hyperlink
Other Designations:
monocyte chemoattractant protein-1,monocyte chemotactic protein 1,small inducible cytokine A2,small inducible gene JE