Synonym:PapainaseProperties:
RelatedCategories | 3.4.x.xPeptidases,3.x.x.xHydrolases,Antibodies,AntibodyCharacterizationandAnalysis,AntibodyFragmentation, |
form | lyophilizedpowder |
molwt | molwt21 kDa |
FeaturedIndustry | DiagnosticAssayManufacturing |
storagetemp. | −20°C |
Description:
Application
UsedtoproduceFabfragmentsofantibodies.Alsousedforcelldissociationsinceithasbeenshowntobemoreeffectiveandlessdamagingwithcertaintissues.
Papain,productP4762frompapayalatex,hasbeenusedtodigestcarotidarterysmoothmusclecells.Papainisusedindissectingsolutions.ItisusedtoproduceFabfragmentsofantibodies.Itisusedforcelldissociationsinceithasbeenshowntobemoreeffectiveandlessdamagingwithcertaintissues.
Biochem/physiolActions
Acysteineproteasethatcleavespeptidebondsofbasicaminoacids,leucine,orglycine.
pHoptimum6.0-7.0
Alsohydrolyzesestersandamides.
PapainpapayalatexhasantifungalactivityagainstC.albicans.Itisacysteineproteasethatcleavespeptidebondsofbasicaminoacids,leucine,orglycine.
.ThepHoptimumis6.0-7.0
.Papainhydrolyzesestersandamides.
OtherNotes
Viewmoreinformationonpapainatwww.Sigma-Aldrich.com/enzymeexplorer.Packaging
1,5ginglassbottle
50,100,500mginglassbottle
25mginpolybottle
PreparationNote
2×Crystallized
Physicalform
Lyophilizedpowdercontainingsodiumacetate
Safety:
Symbol
GHS07,GHS08
Hazardstatements
H315-H319-H334-H335
Precautionarystatements
P261-P305+P351+P338-P342+P311
PersonalProtectiveEquipment
dustmasktypeN95(US),Eyeshields,Faceshields,Gloves
RIDADR
NONHforallmodesoftransport