The antiserum was produced against synthesized phospho-peptide derived from human VE-Cadherin around the phosphorylation site of Tyr731.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
VE-Cadherin (Phospho-Tyr731) Antibody detects endogenous levels of CDH5 only when phosphorylated at Tyr731.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CDH5
Database Links
Gene ID: 1003 Swiss-Prot #: P33151
OMIM #: 601120 Unigene #: Hs.76206
Target Modification
Phospho
Modification Sites
H:Y731, M:Y731
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using VE-Cadherin (Phospho-Tyr731) Antibody
Image 2
Western blot analysis of lysates from HepG2 cells treated with Na3VO4 0.3mM 40', using VE-Cadherin (Phospho-Tyr731) Antibody.