The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
TOP2A (Phospho-Thr1343) Antibody
Synonyms
alpha isozyme; DNA topoisomerase II; DNA topoisomerase II-alpha; P11388-1; TOP-2; TOP2; TOP2A; TP2A
Immunogen
The antiserum was produced against synthesized phospho-peptide derived from human TOP2A around the phosphorylation site of Thr1343.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
TOP2A (Phospho-Thr1343) Antibody detects endogenous levels of TOP2A only when phosphorylated at Thr1343.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
TOP2A
Database Links
Gene ID: 7153 Swiss-Prot #: P11388
OMIM #: 126430 Unigene #: Hs.156346
Target Modification
Phospho
Modification Sites
H:T1343
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using TOP2A (Phospho-Thr1343) Antibody
Image 2
Western blot analysis of lysates from HepG2 cells treated with Ca2+ 40nM 30', using TOP2A (Phospho-Thr1343) Antibody.