The antiserum was produced against synthesized phospho-peptide derived from human PKC theta around the phosphorylation site of Ser695.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
PKC theta (Phospho-Ser695) Antibody detects endogenous levels of PRKCQ only when phosphorylated at Ser695.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PRKCQ
Database Links
Gene ID: 5588 Swiss-Prot #: Q04759
OMIM #: 600448 Unigene #: Hs.498570
Target Modification
Phospho
Modification Sites
H:S695
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PKC θ (Phospho-Ser695) Antibody
Image 2
Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 15', using PKC theta (Phospho-Ser695) Antibody.