The antiserum was produced against synthesized phospho-peptide derived from human p27 Kip1 around the phosphorylation site of Thr187.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
p27 Kip1 (Phospho-Thr187) Antibody detects endogenous levels of CDKN1B only when phosphorylated at Thr187.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CDKN1B
Database Links
Gene ID: 1027 Swiss-Prot #: P46527
OMIM #: 600778/610755 Unigene #: Hs.238990
Target Modification
Phospho
Modification Sites
H:T187, M:T187, R:T187
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using p27 Kip1 (Phospho-Thr187) Antibody
Image 2
Western blot analysis of lysates from HeLa cells treated with EGF or IFN-α, using p27 Kip1 (Phospho-Thr187) Antibody.