The antiserum was produced against synthesized phospho-peptide derived from human MUNC-18a around the phosphorylation site of Ser313.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
MUNC-18a (Phospho-Ser313) Antibody detects endogenous levels of STXBP1 only when phosphorylated at Ser313.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
STXBP1
Database Links
Gene ID: 6812 Swiss-Prot #: P61764
OMIM #: 602926/612164 Unigene #: Hs.288229
Target Modification
Phospho
Modification Sites
H:S313, M:S313, R:S313
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MUNC-18a (Phospho-Ser313) Antibody
Image 2
Western blot analysis of lysates from COS7 cells treated with PMA 125ng/ml 30', using MUNC-18a (Phospho-Ser313) Antibody.