The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
LCK (Phospho-Ser59) Antibody
Synonyms
kinase Lck; LCK; LSK; LSK-T; lymphocyte-specific protein tyrosine kinase; P56-LCK; T cell-specific protein-tyrosine kinase
Immunogen
The antiserum was produced against synthesized phospho-peptide derived from human LCK around the phosphorylation site of Ser59.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
LCK (Phospho-Ser59) Antibody detects endogenous levels of LCK only when phosphorylated at Ser59.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
LCK
Database Links
Gene ID: 3932 Swiss-Prot #: P06239
OMIM #: 153390 Unigene #: Hs.470627
Target Modification
Phospho
Modification Sites
H:S59, M:S59, R:S59
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using LCK (Phospho-Ser59) Antibody
Image 2
Western blot analysis of lysates from Jurkat cells treated with IFN 2500U/ML 30', using LCK (Phospho-Ser59) Antibody.