The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
GRK2 (Phospho-Ser685) Antibody
Synonyms
ADRBK1; ARBK1; BARK; BARK1; beta-adrenergic receptor kinase 1; beta-ARK-1; G protein receptor kinase 2; G- protein coupled receptor kinase 2; kinase GRK2
Immunogen
The antiserum was produced against synthesized phospho-peptide derived from human GRK2 around the phosphorylation site of Ser685.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
GRK2 (Phospho-Ser685) Antibody detects endogenous levels of ADRBK1 only when phosphorylated at Ser685.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
ADRBK1
Database Links
Gene ID: 156 Swiss-Prot #: P25098
OMIM #: 109635 Unigene #: Hs.83636
Target Modification
Phospho
Modification Sites
H:S685, M:S685, R:S685
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using GRK2 (Phospho-Ser685) Antibody
Image 2
Western blot analysis of lysates from HT29 cells treated with insulin 0.01U/ml 15', using GRK2 (Phospho-Ser685) Antibody.