The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
CDC2 (Phospho-Thr161) Antibody
Synonyms
CDC28; CDC2A; CDK1; cell division control protein 2 homolog; cyclin-dependent kinase 1; kinase Cdc2; MPF; p34 protein kinase
Immunogen
The antiserum was produced against synthesized phospho-peptide derived from human CDC2 around the phosphorylation site of Thr161.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
CDC2 (Phospho-Thr161) Antibody detects endogenous levels of CDK1 only when phosphorylated at Thr161.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CDK1
Database Links
Gene ID: 983 Swiss-Prot #: P06493
OMIM #: 116940 Unigene #: Hs.334562
Target Modification
Phospho
Modification Sites
H:T161, M:T161, R:T161
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CDC2 (Phospho-Thr161) Antibody
Image 2
Western blot analysis of lysates from HeLa cells, using CDC2 (Phospho-Thr161) Antibody.