The antiserum was produced against synthesized phospho-peptide derived from human Tuberin around the phosphorylation site of Ser1254.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 ELISA: 1:10000
Specificity
Tuberin (Phospho-Ser1254) antibody detects endogenous levels of Tuberin only when phosphorylated at Ser1254.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Tuberin (Phospho-Ser1254) Antibody
Image 3
Immunohistochemistry analysis of phospho-Thrsc2 (Phospho-Ser1254) antibody in paraffin-embedded human prostate carcinoma tissue.