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Millipore/NS830 | GFAP ELISA Kit/NS830/1 kit96 wells
  • Millipore/NS830 | GFAP ELISA Kit/NS830/1 kit96 wells

Millipore/NS830 | GFAP ELISA Kit/NS830/1 kit96 wells

價格: ￥6288.00 市場價: 10480.00
貨號: NS830
品牌: Millipore
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    • Description
      CatalogueNumberNS830
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionGFAPELISAKit
      OverviewBackground
      GlialFibrillaryAcidicProtein(GFAP)isaclass-IIIintermediatefilamentuniquelyandubiquitouslyexpressedaspartofthestructuralnetworkinastrocyticglialcells.ThisspecificexpressionmakesGFAPidealasabroadastrocyticMarkerinvertebrates.Astrocytesplayavarietyofkeyrolesinsupporting,guiding,nurturing,andsignalingneuronalarchitectureandactivityinahighlyinterdependentmanner.CurrentunderstandingofCNSstructuresuggeststhatthereareatleastasmanyglialcellsastheestimated100billionneurons,butperhapsupwardsof100timesmore.Thus,
      thehealthofbothneuronsandastrocytesisoftenaffectedindiseaseandtrauma.GFAPisprovingtobeausefulbiomarkerinthedetectionofneuraldegeneration.AlthoughdefectsinGFAPhavebeenreportedtocausecertainrareconditions,suchasAlexander’sdisease(Brenneretal.,2001),theobservedinterconnectedphysiologyofneuronalandglialcellshasenabledtheuseofGFAPasaneurodegenerationdetectiongauge.Thisrelationshipisbeingexploitedbyresearchersandcliniciansmeasuringavarietyofneurologicalissuesfromcancertotrauma.GFAPhasbeenusedasabiomarkerintheearlydetectionofchemicallyinducedcancer(Capoetal.,1997)andclassicalglioblastomas(Jungetal.,2007).ElevatedlevelsofGFAPhavebeenreportedintraumaticbraininjury(TBI)andappeartodirectlycorrelatewiththeseverityofinjuryandbraintissueoxygenationincombatcasualtycareresearch(CCR,2008)aswellascivilianaccidents(Mondelloetal.,2010).GFAPhasalsobeenidentifiedasagoodbiomarkerinstudiesoftoxininducedacutebraininjury(Liaoetal.,2008),indifferentiationofhemorrhagicvs.ischemicstrokes(Foerchetal.,2006),aswellasincardiacarrest(Kanekoetal.,2009).MilliporenowoffersanELISAassayforsensitivemeasurementofGFAPproteinintissuehomogenatesandBIOLOGicalfluids(serum,plasma,CSF,andserumfreemedia)fromhuman,mouse,andrat.

      PrincipalsofProcedure
      ThisassayisaSandwichELISAbasedsequentiallyon:1)captureofsolubleGFAPproteintothewellsofamicrotiterplatecoatedbyapre-titeredamountofanti-GFAPmonoclonalantibody;2)removalofunboundmaterialbywashing;3)bindingofbiotinylatedanti-GFAPmonoclonalantibodyto
      thepreviouslycapturedGFAPprotein;4)removalofunboundmaterialbywashing;5)bindingofstreptavidinconjugatedhorserADIshperoxidasetothebiotinylatedantibodies;6)removalofexcessorunboundenzymeconjugatesbywashing;and7)quantificationofimmobilizedantibody-enzyme
      complexesbymonitoringhorseradishperoxidaseactivityinthepresenceofthesubstrate3,3’,5,5’-tetramethylbenzidine(TMB).Enzymeactivityismeasuredspectrophotometricallybyrecordingabsorbancereadingsat450nm.Increasesinabsorbencyaredirectlyproportionaltotheamountof
      GFAPproteincapturedfromthesample.ThereforetheproteinconcentrationofunknownscanbederivedbyinterpolationusingastandardcurvegeneratedinthesameassaywithreferencestandardsofknownconcentrationsofGFAP.
      AlternateNames
      • GFAP
      • GlialFibrillaryAcidicProteinELISA
      MaterialsRequiredbutNotDelivered1.Absorbentpaperorcloth
      2.Conventional,multi-channel,orrepeatingPipettes
      3.Reagentreservoirs
      4.Orbitalmicrotiterplateshaker/washer(optional)
      5.GraduatedSEROlogicalpipettes
      6.Microtiterplatereadercapableofreadingabsorbencyat450nm
      7.Graphingsoftwareforplottingdataorgraphpaperformanualplottingofdata
      8.Microfugetubesforstandardandsampledilutions
      9.Mechanicalvortex
      10.Milli-Q?Water
      ProductInformation
      Components
      • 96-WellAnti-GFAPCoatedPlate(CS206352):1Plate
      • 1XAssayBuffer(CS206357):40mL
      • 10XWashBuffer(CS206360):50mL
      • GFAPProteinStandard(CS206361):1Vial
      • GFAPQualityControl#1(CS206351):1Vial
      • GFAPQualityControl#2(CS206475):1Vial
      • BiotinylatedAnti-GFAPDetectionAntibody(CS206356):12mL
      • EnzymeSolution(CS206358):12mL
      • TMBSolution(CS202682):10mL
      • StopSolution(60193):12mL
      • PlateSealers(CS200443):10Sheets
      DetectionmethodChromogenic
      StorageandShippingInformation
      StorageConditionsStorage:TheGFAPELISAkitisshippedoniceandshouldbestoredat2-8°Cuponarrival.
      StABIlity:Kitcomponentsarestableforaminimumof6monthsfromthedateofreceiptifstoredand
      handledasdescribedabove.
      Applications
      KeyApplications
      • ELISA
      BiologicalInformation
      SpeciesReactivity
      • Mouse
      • Rat
      • Human
      AnalytesAvailable
      • GFAP
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesoneofthemajorintermediatefilamentproteinsofmatureastrocytes.Itisusedasamarkertodistinguishastrocytesfromotherglialcellsduringdevelopment.MutationsinthisgenecauseAlexanderdisease,araredisorderofastrocytesinthecentralnervoussystem.Alternativesplicingresultsinmultipletranscriptvariantsencodingdistinctisoforms.
      GeneSymbol
      • GFAP
      UniProtNumber
      UniProtSummaryFUNCTION:GFAP,aclass-IIIintermediatefilament,isacell-specificmarkerthat,duringthedevelopmentofthecentralnervoussystem,distinguishesastrocytesfromotherglialcells.
      SUBCELLULARLOCATION:Cytoplasm.Note:Associatedwithintermediatefilaments.
      TISSUESPECIFICITY:Expressedincellslackingfibronectin.
      PTM:PhosphorylatedbyPKN1.
      SIMILARITY:Belongstotheintermediatefilamentfamily.
      PhysicochemicalInformation
      Sensitivity
      • 1.5ng/mL
      StandardCurveRange
      • 1.5to100ng/mL
      Dimensions
      MaterialsInformation
      MaterialsInformation
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