Sigma-Aldrich/SILu™Lite SigmaMAb Universal Antibody Standard human/MSQC4-1MG/1MG

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Properties:

Description:

Analysis Note

SigmaMab Heavy Chain
EVQLVESGGGLVQPGGSLRLSCVASGFTLNNYDMHWVRQGIGKGLEWVSKI
GTAGDRYYAGSVKGRFTISRENAKDSLYLQMNSLRVGDAAVYYCARGAGRW
APLGAFDIWGQGTMVTVSS|ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYF
PEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVN
HKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISR
TPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRV
VSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPS
RDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFL
YSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPG

SigmaMab Light Chain
QSALTQPRSVSGSPGQSVTISCTGTSSDIGGYNFVSWYQQHPGKAPKLMIY
DATKRPSGVPDRFSGSKSGNTASLTISGLQAEDEADYYCCSYAGDYTPGV
VFGGGTKLTVL|GQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTV
AWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQ
VTHEGSTVEKTVAPTECS

Also available as a stable isotope-labled product, Silu^™Mab (Product Number MSQC3)

Package size based on protein content determined by A₂₈₀

Features and Benefits

Calculated molecular weight values of the SigmaMAb light chains, heavy chains, and intact protein, with the most abundant glycoforms, are as follows:

Description / Composition / Modification / Average Mass (Da)

Light chain, reduced / C₁₀₀₆H₁₅₅₅N₂₆₇O₃₃₃S₇ / Pyroglutamic acid (Q) / 22942.2

Heavy chain, reduced / C₂₁₈₁H₃₃₉₃N₅₈₇O₆₆₃S₁₆ / (no modification) / 48957.8
C₂₂₃₇H₃₄₈₅N₅₉₁O₇₀₂S₁₆ / G0F / 50403.2
C₂₂₄₃H₃₄₉₅N₅₉₁O₇₀₇S₁₆ / G1F / 50565.3
C₂₂₄₉H₃₅₀₅N₅₉₁O₇₁₂S₁₆ / G2F / 50727.5

Native intact mass, non-reduced / C₆₃₇₄H₉₈₆₄N₁₇₀₈O₁₉₉₂S₄₆ / 2 X Pyroglutamic acid (Q) / 143767.7
C₆₄₈₆H₁₀₀₄₈N₁₇₁₆O₂₀₇₀S₄₆ / G0F+G0F / 146658.4
C₆₄₉₂H₁₀₀₅₈N₁₇₁₆O₂₀₇₅S₄₆ / G0F+G1F / 146820.6
C₆₄₉₈H₁₀₀₆₈N₁₇₁₆O₂₀₈₀S₄₆ / G1F+G1F / 146982.7
C₆₅₀₄H₁₀₀₇₈N₁₇₁₆O₂₀₈₅S₄₆ / G1F+G2F / 147144.8
C₆₅₁₀H₁₀₀₈₈N₁₇₁₆O₂₀₉₀S₄₆ / G2F+G2F / 147307.0

Physical form

Supplied as a lyophilized powder containing phosphate buffered saline

General description

SILU^™ Lite SigmaMAb is a recombinant human monoclonal IgG1 lambda light antibody with a molecular mass of ∼150 kDa expressed in CHO cells. It is designed for optimization of accurate intact mass analysis of monoclonal antibodies, biosimilars, and pharmaceutical products. Accurate intact mass analysis of such large biomolecules can provide comprehensive information about structural and post-translational modifications such as glycosylation. Other information such as heterogeneity, batch-to-batch variation, amino acid truncation, and N-terminal Lys processing, aggregation, and degradation can be determined. Intact mass analysis using mass spectrometry is also very important for formulation and storage in therapeutic monoclonal antibody drug development.

Legal Information

SILu is a trademark of Sigma-Aldrich Co. LLC

Safety: