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CompatIBLewithhigh-throughputhandlingsystems.Canbeadaptedforusewithcuvettes.
Thekitisshippedatroomtemperature.StoreReagentAat–20°C.Storeallotherreagentsat2-8°C.
Chromium,anessentialtraceelement,isatransitionmetalthatprimarilyexistsintwostableoxidationstates,hexavalent[Cr(VI)]andtrivalent[Cr(III)].Chromiumiswidelyusedinvariousindustriessuchaselectroplating,leathertanning,chromepaint,dying,hardenedsteel,ceramic,andglass.Cr(VI)isproducedalmostexclusivelybyindustrialprocesses;whereas,innaturechromiumprimarilyexistsinitstrivalentform.Cr(III)isgenerallyregardedasnontoxicduetoitspoorabsorption.Cr(VI)isconsideredapulmonarycarcinogenandhastestedpositiveingenotoxicitytests.Cr(VI)isconsideredaseriouspollutantinmanywaterstreamsduetoitscarcinogenicpotential.Intraceamounts,Cr(III)mayberequiredforglucoseandlipidmetabolism.Inthiskit,chromiumisdeterminedinasimple,one-stepassayinwhichCr(VI)formsaspecificcomplexwithachromgenicdye,resultinginacolorimetric(480nm)product,proportionaltothechromiumpresent.Cr(III)canbeconvertedtoCr(VI)withnitricacid/hydrochloricacid,allowingthedeterminationofCr(III)and/ortotalchromium[Cr(III)+Cr(VI)].
Thiskitissuitableforthedetectionofchromiuminbiological,environmental,food,andbeveragesamples.
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