Synonym:FRASCAssay,FerricReducing/AntioxidantandAscorbicAcidProperties:
RelatedCategories | BloodandUrineChemistryAssayKits,CellBIOLOGy,EnzymaticKitsfortheQuantitationofNutrientsandMetabolites,MetabolismAssayKitsA-Z,Metabolomics, |
usage | sufficientfor100 colorimetric?tests |
storagetemp. | −20°C |
Description:
FrequentlyAskedQuestions
FrequentlyAskedQuestionsareavailableforthisProduct.
Generaldescription
AscorbicAcid,alsoknownasVitaminC,isasix-carbonlactoneproducedbyplantsandsomeanimalspeciesbutnotbyhumansandotherprimates.Ascorbicacidfunctionsasanenzymaticcofactorformultipleenzymes,servingasanelectrondonorformonooxygenasesanddioxygenases.Ascorbicacidalsofunctionsasapowerfulantioxidant,particularlyinregardstoreactiveoxygenspecies.
Inthisassay,ascorbicacidconcentrationisdeterminedusingtheFerricReducing/AntioxidantandAscorbicAcid(FRASC)assay.Inthisassay,Fe3+isreducedtoFe2+byantioxidantspresentinthesample,whichresultsinacolorimetric(593nm)product.Theadditionofascorbateoxidasetoparallelsamplesoxidizesanyascorbicacidpresentallowingforthemeasurementoftheascorbicacidconcentration.
Thiskitissuitableforusewithcellandtissueculturesupernatantsaswellasserum,plasma,urine,andotherbiologicalfluids.
Safety:
RIDADR
NONHforallmodesoftransport