OxaloacetateTransaminaseActivityAssayKitProperties:
RelatedCategories | AminoAcidMetabolismAssayKits,BloodandUrineChemistryAssayKits,CellBIOLOGy,EnzymaticKitsfortheQuantitationofNutrientsandMetabolites,MetabolismAssayKitsA-Z, |
usage | sufficientfor100 colorimetric?tests |
storagetemp. | −20°C |
GeneInformation | human...GOT1(2805) mouse...GOT1(14718) rat...GOT1(24401) |
Description:
FeaturesandBenefits
CompatIBLewithhigh-throughputhandlingsystems.
Generaldescription
AspartateAminotransferase(AST),formerlycalledserumglutamate-oxaloacetatetransaminase(SGOT),isapyridoxalphosphate(PLP)-dependentenzymethatcatalyzestheconversionofaspartateandα-ketoglutaratetooxaloacetateandglutamate.SimilartoAlanineAminotransferase(ALT),ASTlevelsinbloodarecommonlyusedasaMarkerforliverfunction.However,ASThasabroadertissuedistributionthanALTandperturbationsinASTlevelscanoccurinresponsetodiseasesorinjuriesinmultipletissuesincludingskeletalandheart.
TheAspartateAminotransferaseActivityAssaykitprovidesasimpleanddirectprocedureformeasuringASTactivityinavarietyofsamples.Inthiskit,thetransferofanaminogroupfromaspartatetoα-ketoglutarateresultsinthegenerationofglutamate,resultingintheproductionofacolorimetric(450nm)productproportionaltotheASTenzymaticactivitypresent.OneunitofASTistheamountofenzymethatwillgenerate1.0μmoleofglutamateperminuteatpH8.0at37°C.
Safety:
RIDADR
NONHforallmodesoftransport