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Thelipasefamilyofenzymescatalyzethecleavageoftheesterbondsoflipids.Inmammals,thisfamilyincludesmanycriticalmembersincludingpancreatic,hepatic,endothelial,andlipoproteinlipase.Lipases,suchaspancreaticlipase,arecriticalforthemetabolismoflipids.Lipasesalsoplaykeyrolesinprocessessuchascellsignalingandinflammation.Measurementsoflipaseactivityarecommonlyusedtoscreenforpancreaticinjuryordisease,andtomonitordiseasessuchascysticfibrosis,celiacdisease,andCrohn′sdisease.TheLipaseActivityAssaykitprovidesasimpleanddirectprocedureformeasuringlipaseactivityinavarietyofsamples.Lipaseactivityisdeterminedusingacoupledenzymereaction,whichresultsinacolorimetric(412nm)productproportionaltotheenzymaticactivitypresent.OneunitofLipaseistheamountofenzymethatwillgenerate1.0μmoleofTNBperminuteat37°C.
CompatIBLewithhigh-throughputhandlingsystems.
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