Properties:
RelatedCategories | BloodandUrineChemistryAssayKits,CarbohydrateMetabolismAssayKits,CellBIOLOGy,EnzymaticActivityAssayKits,EnzymaticKitsfortheQuantitationofNutrientsandMetabolites, |
usage | sufficientfor100 colorimetric?tests |
storagetemp. | −20°C |
GeneInformation | human...AMY1A(278) |
Description:
Generaldescription
Amylaseenzymesareglycosidehydrolases,whichcleaveglucanlinkagesinpolysaccharidessuchasstarchorglycogentoreleasepolysaccharidemolecules.α-Amylaseisfoundinanimals,plants,andmanyfungi.Inmammals,α-amylaseisthepredominantformofamylaseandisoneofthemajorenzymesofdietarydigestion.BoththeactivityandthestABIlityofα-amylaseisdependentoncalciumions.Inhumans,α-amylaselevelsarehighestinpancreaticandsalivarysecretions.Increasedbloodandurinelevelsofamylaseoccurduetopancreaticdisordersandsalivarytrauma.IncreasedsalivaryamylaseactivityisalsoaMarkerforincreasedsympatheticnervoussystemactivity.
TheAmylaseActivityAssaykitquantifiesamylaseactivityinavarietyofbiologicalsamples.Theassayisquick,convenient,andsensitive.Inthiskit,amylaseactivityisdeterminedusingacoupledenzymaticassay,whichresultsinacolorimetric(405nm)product,proportionaltotheamountofsubstrate,ethylidene-pNP-G7,cleavedbytheamylase.Oneunitistheamountofamylasethatcleavesethylidene-pNP-G7togenerate1.0μmoleofp-nitrophenolperminuteat25°C.
Suitability
Suitableforthequantificationofamylaseactivityinavarietyofbiologicalsamples.
Safety:
RIDADR
NONHforallmodesoftransport