Properties:
RelatedCategories | AminoAcidMetabolismAssayKits,BloodandUrineChemistryAssayKits,CellBIOLOGy,EnzymaticKitsfortheQuantitationofNutrientsandMetabolites,MetabolismAssayKitsA-Z, |
usage | sufficientfor100 fluorometric?tests |
storagetemp. | −20°C |
Description:
Generaldescription
Phenylalanineisanon-polaressentialaminoacid.Intheliver,phenylalanineisconvertedtotyrosine,whichisaprecursorformultiplecompoundsincludingL-DOPAandmelanin.Defectsintheactivityofphenylalaninehydroxylaseresultintheinheritedmetabolicdisorderphenylketonuria(PKU).PKUresultsinthebuildupofphenylalanineandphenylalaninemetabolites,andcanresultingrowthdefectsandmentalretardationifnottreated.
Phenylalanineconcentrationisdeterminedbyacoupledenzymeassay,whichresultsinthedeaminationofphenylalanineandtheproductionofNADHwhichreactswiththeproberesultinginafluorescent(λex=535nm/λem=587nm)product,proportionaltothephenylalaninepresent.
SuitABIlity
SuitableforL-phenylalaninedetectionincellandtissueculturesupernatants,serum,andotherbiologicalsamples
Safety:
RIDADR
NONHforallmodesoftransport