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Millipore/MAB5278 | Anti-Tryptophan Hydroxylase/Tyrosine Hydroxylase/Phenylalanine Hydroxylase Antibody, clone PH8/MAB52
  • Millipore/MAB5278 | Anti-Tryptophan Hydroxylase/Tyrosine Hydroxylase/Phenylalanine Hydroxylase Antibody, clone PH8/MAB52

Millipore/MAB5278 | Anti-Tryptophan Hydroxylase/Tyrosine Hydroxylase/Phenylalanine Hydroxylase Antibody, clone PH8/MAB52

價格: ￥4308.00 市場價: 7180.00
貨號: MAB5278
品牌: Millipore
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    • Description
      CatalogueNumberMAB5278
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-TryptophanHydroxylase/TyrosineHydroxylase/PhenylalanineHydroxylaseAntibody,clonePH8
      BackgroundInformationPH8AntibodyisamurinemonoclonalantibodythatbindsacommonepitopeofTryptophanhydroxylase(TRH),Tyrosinehydroxylase(TYH)andPhenylalaninehydroxylase(PAH).1IntheliteraturethismonoclonalantibodyiscitedasPH8.Tyrosinehydroxylase(TYH)istheenzymewhichconvertstyrosinetodihydroxyphenylalanine(L-dopa),aprecursorofthecatecholamineneurotransmittersdopamine,noradrenalineandadrenaline.Tryptophanhydroxylase(TRH)istheenzymethatconverts5-hydroxytryptophantoSEROtonin.InfreshtissuethePH8AntibodybindstoTYHandTRHsoisaMarkerforTYH-containingcatecholaminergicneurons,2andserotonergicneurons.3,4,5Tryptophanhydroxylase(TRH)canbeusedasamarkerforserotoninasitconverts5-hydroxy-tryptophantoserotonin.Serotoninisrapidlymetabolisedandisunabletobedetectedbyanti-serotoninantibodiesinpostmortemtissue.Inhumantissuethathasbeenformalin-fixed,duetoachangeintheantigenicdeterminantoftyrosinehydroxylase(TYH),PH8Antibodywillbindonlytryptophanhydroxylase(TRH).3,4,5ThePH8Antibodycanthereforebeusedtospecificallyidentifyserotonergicneuronsinfixedhumantissue.Phenylalaninehydroxylase(PAH)canbedetectedinhepatictissuesectionsusingthePH8Antibody.
      ProductInformation
      FormatPurified
      PresentationTheimmunoglobulinfractionhasbeenpurifiedfromascitesfluidbyProteinGchromatography.500μgliquidproteinat2mg/mLinphosphatebufferedsaline(PBS).Theproductissuppliedsterile-filteredthrougha0.22micronfilter.
      StorageandShippingInformation
      StorageConditionsThePH8Antibodyisshippedliquidatambienttemperature.Liquidmaterialisstableforatupto6monthswhenstoredat2-8°C.
      Applications
      ApplicationThisAnti-TryptophanHydroxylase/TyrosineHydroxylase/PhenylalanineHydroxylaseAntibody,clonePH8isvalidatedforuseinIHC,IP,WBforthedetectionofTryptophanHydroxylase/TyrosineHydroxylase/PhenylalanineHydroxylase.
      KeyApplications
      • Immunohistochemistry
      • Immunoprecipitation
      • WesternBlotting
      ApplicationNotesThePH8Antibodycanbeusedtoidentifydopaminergicandserotonergicneuronsbyimmuno-histochemistryandforWesternblotanalysis,immunoprecipitationandimmuno-histochemistryofTYH,PAHandTRH.

      PROTOCOLS

      Immunohistochemistry

      ThePH8Antibodycanbeusedfortheimmunohistochemicaldetectionofdopaminergicandserotonergicneuronsinhumanandratbrainstemtissue.

      1.Tissueshouldbeformalinfixedandstoredinformalinpriortouseforaminimumoffivedays.

      2.Cryoprotecttissueusing30%sucrosein0.1MTrispH7.4bufferfor24-72hours.

      3.Cutusingasledgemicrotometo50μmthickness.

      4.WashthetissuesamplesusingTrisbufferpriortocommencingthestainingprocedure.

      5.Treatthetissuesamplesfor3x15minutesin50%alcohol.

      6.Treatthetissuesamplesfor20minutesin50%alcoholand3%H2O2.

      7.Treattissuefor20minuteswith10%normalhorseseruminTrisbuffer.ThisactstoblockendogeneousH2O2staining.

      8.DilutethePH8AntibodyinTrisbuffer,addtothetissuesamplesandincubatefor1-3days.Recommendeddilutionsare1:2,000-1:10,000.Athighantibodyconcentrationallmonoaminergicneuronsarestainedwithnodistinctionbetweenserotonegicandcatecholominergiccells.Bydilutingtheantibodyconcentrate,celltypesaredistinguishableduetovariationinstainingintensity.Atlowerconcentrations(1:5,000-1:10,000dilution)onlyserotonergiccellswillstain.

      9.Washtissue(3x15minutes),thenaddabiotinylatedanti-mousesecondaryantibodyandincubateonanorbitalshakeratroomtemperature(RT)for1hour.

      10.Washtissue(3x15minutes)andincubateonanorbitalshakeratRTfor1hourwiththetertiarycomplex(ELITEKIT,Vector,USA).

      11.Washtissue(3x15minutes)andincubateonanorbitalshakeratRTfor10minuteswithTrisbuffereddiamino-benzidinesubstrate.Add0.1%H2O2andincubateonanorbitalshakeratRTforafurther5minutes.

      12.Mounttissueontogelatinisedslidesandallowtodrypriortomicroscopy.
      BIOLOGicalInformation
      ClonePH8
      HostMouse
      IsotypeIgG1
      SpeciesReactivity
      • Human
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesamemberofthepterin-dependentaromaticacidhydroxylasefamily.Theencodedproteincatalyzesthefirstandratelimitingstepinthebiosynthesisofserotonin,animportanthormoneandneurotransmitter.Thehumangenomecontainstworelatedtryptophanhydroxylases,oneonchromosome11p15-p14andoneonchromosome12q21.Thisgeneisexpressedpredominantlyinthegut,pinealgland,spleen,andthymus.
      GeneSymbol
      • TPH1
      • TPRH
      • MGC119994
      • TPH
      • TRPH
      • EC1.14.16.4
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P07101#Playsanimportantroleinthephysiologyofadrenergicneurons.
      COFACTOR:Fe(2+)ion.
      SIZE:528aminoacids;58524Da
      TISSUESPECIFICITY:Mainlyexpressedinthebrainandadrenalglands.
      DISEASE:SwissProt:P07101#DefectsinTHarethecauseofautosomalrecessiveSegawasyndrome[MIM:605407];alsoknownasDOPA-responsivedystonia.Typically,itbeginsinchildhoodoradolescencewithprogressivedifficultyinwalkingand,insomecases,spasticity.Somecasespresentwithparkinsoniansymptomsininfancyandarereferredtoasautosomalrecessiveinfantileparkinsonism.
      SIMILARITY:SwissProt:P07101##Belongstothebiopterin-dependentaromaticaminoacidhydroxylasefamily.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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