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Millipore/MAB5258-20UG | Anti-Synaptophysin Antibody, clone SY38/MAB5258-20UG/20 µg
  • Millipore/MAB5258-20UG | Anti-Synaptophysin Antibody, clone SY38/MAB5258-20UG/20 µg

Millipore/MAB5258-20UG | Anti-Synaptophysin Antibody, clone SY38/MAB5258-20UG/20 µg

價(jià)格: ￥3576.00 市場(chǎng)價(jià): 5960.00
貨號(hào): MAB5258-20UG
品牌: Millipore
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    • Description
      CatalogueNumberMAB5258-20UG
      Replaces04-1019
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-SynaptophysinAntibody,cloneSY38
      AlternateNames
      • synaptophysin
      • Synaptophysin
      BackgroundInformationSynaptophysinisa38kDaglycoproteinpresentinthemembraneofneuronalpresynapticvesiclesinbrain,spinalcord,retina,vesiclesofadrenalmedulla,neuromuscularjunctions,andendocrinecells.Itisalsoexpressedbyneuroendocrinecellsthroughoutthebody,bothnormalandneoplastic.SynaptophysinisausefulMarkerfortheidentificationofnormalneuroendocrinecellsandneuroendocrineneoplasms.Thisantibodyreactswithneuroendocrinecellsofhumanadrenalmedulla,carotidbody,skin,pituitarygland,thyroid,lung,pancreas,andgastointestinalmucosa.Italsoreactswithawidespectrumofneuroendocrineneoplasmsofneuraltypeincludingneuroblastomas,ganglioneuroblastomas,ganglioneuromas,pheochromocytomas,chromaffin,andnonchromaffinparagangliomas.
      ProductInformation
      FormatPurified
      Control
      • PositiveControl:Pancreastissue
        NegativeControl:Normalmouseserum
        Rathippocampustissue,mousebrainlysate.
      PresentationPurifiedmousemonoclonalIgG1κinbuffercontaining20mMsodiumphosphatebuffer,0.25MNaCl,containing0.1%sodiumazide.
      StorageandShippingInformation
      StorageConditionsStablefor6monthsat2-8oCfromdateofreceipt.DONOTFREEZE.
      Applications
      ApplicationDetectSynaptophysinusingthisAnti-SynaptophysinAntibody,cloneSY38validatedforuseinFC,IC,IH&WB.
      KeyApplications
      • FlowCytometry
      • Immunocytochemistry
      • Immunohistochemistry
      • WesternBlotting
      ApplicationNotesFlowCytometry(FACS):
      ApreviouslotofthisantibodywasusedinFlowCytometry.

      WesternBlot:
      (Provoda,2000)Reactswitha38kDatransmembraneglycoprotein

      Immunohistochemistry:
      Forrelativelycytoplasm-richneuroendocrinictumorsafinalconcentrationof1μg/mLisrecommended.Concerningcytoplasmdeficienttumorsaconcentrationof2μg/mLshouldbeused.Idealfrozensections(4-5mm)areobtainedfromshock-frozentissuesamples.Thefrozensectionsareair-driedandthenfixedwithacetonefor5-10minat-20°C.Excessacetoneisallowedtoevaporateatroomtemperature.Materialfixedinalcoholorformalinandembeddedinparaffincanalsobeused.
      Itisadvantageoustoblockunspecificbindingsitesbyoverlayingthesectionswithfetalcalfserumfor20-30minatroomtemperature.Excessoffetalcalfserumisremovedbydecantingbeforeapplicationoftheanti-bodysolution.
      Cytocentrifugepreparationsofsinglecellsorcellsmearsarealsofixedinacetone.Thesepreparationsshouldbutshouldbefolloweddirectlybyantibodytreatment.

      Optimalworkingdilutionsmustbedeterminedbyenduser.
      BIOLOGicalInformation
      ImmunogenVesicularfractionofbovinebrain.TheSY38epitopeistoapentapeptiderepeatstructureinthecarboxy-terminalcytoplasmictailofsynaptophysin(Knaus&Betz1990).
      CloneSY38
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityTheantibodyreactswithpresynapticvesiclesofcerebralandspinalneurons,ofneuromuscularendplatesandwiththeretinaofman,cow,ratandmouse.Theantibodyalsoreactswithvesiclesofadrenalmedullaandisletcells,andadditionallyallowsspecificstainingofneuronal,adrenalandneuroepithelialtumors,suchaspheochromocytoma,paraganglioma,isletcelltumors(incl.Insulinoma),medullarythyroidcarcinomaanddiversepulmonaryandgastro-intestinalcarcinoids.Theantibodyalsostainsneurosecretoryvesiclesofcertainculturecells,e.g.oftheratcelllinePC-12.
      IsotypeIgG1κ
      SpeciesReactivity
      • Avian
      • Bovine
      • Fish
      • Human
      • Mouse
      • Rat
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummarySynaptophysin(p38)isanintegralmembraneproteinofsmallsynapticvesiclesinbrainandendocrinecells.[suppliedbyOMIM]
      GeneSymbol
      • SYP
      PurificationMethodProteinAPurfied
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P08247#PossIBLyinvolvedinstructuralfunctionsasorganizingothermembranecomponentsorintargetingthevesiclestotheplasmamembrane.
      SIZE:313aminoacids;33845Da
      SUBUNIT:Homohexamerorhomotetramer.
      SUBCELLULARLOCATION:Cytoplasmicvesicle,secretoryvesicle,synapticvesiclemembrane;Multi-passmembraneprotein.
      TISSUESPECIFICITY:Characteristicofatypeofsmall(30-80nm)neurosecretoryvesicles,includingpresynapticvesicles,butalsovesiclesofvariousneuroendocrinecellsofbothneuronalandepithelialphenotype.
      DOMAIN:SwissProt:P08247Thecalcium-bindingactivityisthoughttobelocalizedinthecytoplasmictailoftheprotein.
      PTM:Ubiquitinated;mediatedbySIAH1orSIAH2andleADIngtoitssubsequentproteasomaldegradation(Bysimilarity).
      SIMILARITY:Belongstothesynaptophysin/synaptobrevinfamily.&Contains1MARVELdomain.
      MolecularWeight38kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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