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Millipore/MAB369 | Anti-Dopamine Transporter Antibody, NT, clone DAT-Nt/MAB369/100 µL
  • Millipore/MAB369 | Anti-Dopamine Transporter Antibody, NT, clone DAT-Nt/MAB369/100 µL

Millipore/MAB369 | Anti-Dopamine Transporter Antibody, NT, clone DAT-Nt/MAB369/100 µL

價(jià)格: ￥3948.00 市場價(jià): 6580.00
貨號(hào): MAB369
品牌: Millipore
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    • Description
      CatalogueNumberMAB369
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-DopamineTransporterAntibody,NT,cloneDAT-Nt
      AlternateNames
      • DAT
      BackgroundInformationThetransmembranedopaminetransporter(DAT)islocatedonthepresynapticnerveterminalandisresponsIBLeforterminatingdopaminergictransmissionbytransportingdopaminefromthesynapticcleftintothedopaminergicneuron(reuptake).Dopaminergicpathwayshavebeenstronglyimplicatedinrewardandaddiction,motivation,alcoholism,ADHDanddegenerativemotordiseasessuchasParkinson"s,Huntington"sandChorea.
      ProductInformation
      FormatCultureSupernatant
      Control
      • PositiveControl:Brain(caudate,putamen,andnucleusaccumbens)tissue
      PresentationUnPurifiedtissueculturesupernatant,filteredthrougha0.2μmmembranepriortovialing.Productcontains20%FBSandCiprofloxacinatfinalconcentrationof10μg/mL.
      StorageandShippingInformation
      StorageConditionsMaintainfor1yearat-20°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
      Applications
      ApplicationThisAnti-DopamineTransporterAntibody,N-terminus,cloneDAT-NtisvalidatedforuseinIC,IH,WBforthedetectionofDopamineTransporter.
      KeyApplications
      • Immunocytochemistry
      • Immunohistochemistry
      • WesternBlotting
      ApplicationNotesWesternblot:Recognizesadiffusebandatapproximately70-85kDaonwesternblotsofextracts(20ugtotalprotein)fromhumancaudate,putamen,andnucleusaccumbens.

      Immunohistochemistry:4%paraformaldehydefixedtissue(careshouldbetakennottoover-fixtissue);perfusionfollowedbylessthan90minutespost-fixation,thencryoprotect.Suggestedworkingdilution1:1,000-1:10,000.Ifusingonrattissue,absorbedanti-ratsecondaryantibodiesarerecommended,andtheuseofratPAPsystems,orABCdetectionwillenhancesensitivity.

      Immunocytochemistry:1:5,000to1:10,000.

      Immunoblotting(notrecommendedforuseonrat)

      Optimalworkingdilutionsandprotocolsmustbedeterminedbyenduser.



      IMMUNOHISTOCHEMISTRYPROTOCOLFORMAB369

      Thisantibodyhasbeenusedsuccessfullyon30μm,freefloating,4%paraformaldehydefixedmousebraintissue.Allstepsareperformedunderconstantagitation.Suggestedprotocolfollows.

      1)3x10minutewashesinTBS(withorwithout0.25%Triton).

      2)Incubatefor30minutesinTBSwith3%serum(sameashostfromsecondaryantibody).

      3)IncubateprimaryantibodydilutedappropriatelyinTBSwith1%serum(sameashostfromsecondaryantibody)(withorwithout0.25%Triton)for2hoursatroomtemperaturefollowedby16hoursat4°C.

      4)3x10minutewashesinTBS.

      5)IncubatewithsecondaryantibodydilutedappropriatelyinTBSwith1%serum(sameashostfromsecondaryantibody).

      6)3x10minutewashesinTBS.

      7)ABCElite(1:200VectorLabs)inTBS.

      8)2x10minutewashesinTBS.

      9)1x10minutewashinphosphatebuffer(nosaline).

      10)DABreactionwith0.06%NiCladdedforintensification.

      11)2x10minutewashesinPBS.

      12)1x10minutewashinphosphatebuffer(nosaline).
      BIOLOGicalInformation
      ImmunogenN-terminusofhumandopaminetransporterfusedtoGlutathioneS-transferase.
      EpitopeN-terminus
      CloneDAT-Nt
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostRat
      SpecificityRecognizesDopaminetransporter,N-terminus.ShowsnocrossreactivitytothecloselyrelatedSEROtoninandnorepinephrinetransporters(Miller,1997).ImmunolocalizationofDATonparaformaldehydefixedfrozensectionsofhumanbrainusingMAB369showsdensepunctatestainingthroughoutthecaudate,putamenandaccumbens(Miller,1997).
      IsotypeIgG2aκ
      SpeciesReactivity
      • Human
      • Mouse
      • Monkey
      • Rat
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThedopaminetransporter(DAT1)mediatestheactivereuptakeofdopaminefromthesynapseandisaprincipalregulatorofdopaminergicneurotransmission.TheDAT1genehasbeenimplicatedinhumandisorderssuchasparkinsonism,Tourettesyndrome,andsubstanceabuse(Vandenberghetal.,1992[PubMed1359373]).[suppliedbyOMIM]
      GeneSymbol
      • SLC6A3
      • DAT1
      • ADHD
      • ADD
      • DAT
      PurificationMethodUnpurified
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:Q01959#Aminetransporter.Terminatestheactionofdopaminebyitshighaffinitysodium-dependentreuptakeintopresynapticterminals.
      SIZE:620aminoacids;68495Da
      SUBUNIT:Homooligomer;disulfide-linked.InteractswithPRKCABPandTGFB1I1.
      SUBCELLULARLOCATION:Membrane;Multi-passmembraneprotein.
      SIMILARITY:SwissProt:Q01959##Belongstothesodium:neurotransmittersymporter(SNF)family.
      MISCELLANEOUS:Thisproteinisthetargetofpsychomotorstimulantssuchasamphetaminesorcocaine.
      MolecularWeight~70-85kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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