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Millipore/MAB3402 | Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5/MAB3402/40 µg
  • Millipore/MAB3402 | Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5/MAB3402/40 µg

Millipore/MAB3402 | Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5/MAB3402/40 µg

價格: ￥3948.00 市場價: 6580.00
貨號: MAB3402
品牌: Millipore
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    • Description
      Catalogue NumberMAB3402
      Replaces04-1031; 04-1062
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Glial Fibrillary Acidic Protein Antibody, clone GA5
      Alternate Names
      • GFAP
      Background InformationGlial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.
      Product Information
      FormatPurified
      Control
      • Mouse brain tissue, Astrocyte culture.
      PresentationPurified mouse monoclonal IgG1 in buffer containing 0.02 M phosphate buffer, 0.25 M NaCl with 0.1% sodium azide, pH 7.6.
      Storage and Shipping Information
      Storage ConditionsStore the reconstituted antibody at 2-8°C for up to 6 months after date of receipt.
      DO NOT FREEZE.
      Applications
      ApplicationAnti-Glial Fibrillary Acidic Protein Antibody, clone GA5 is an antibody against Glial Fibrillary Acidic Protein for use in IC, IH, IH(P) & WB with more than 55 product citations.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunocytochemistry:
      5 μg/mL of a previous lot was used.

      Immunohistochemistry:
      5 μg/mL

      Immunoblotting:
      Recognizes a 51kDa protein in reducing westerns of total brain lysates.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified glial filament (Debus, E., 1983).
      CloneGA5
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThe antibody reacts with GFAP from human, pig, chicken and rat. In tissue sections this antibody stains astrocytes and Bergman glia cells (Debus, E., 1983).
      IsotypeIgG1
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Pig
      • Chicken
      • Bovine
      • Rabbit
      Species Reactivity NoteHuman, mouse, and rat. Expected to cross-react with porcine, chicken, bovine, and rabbit.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant has been described, but its full length sequence has not been determined.
      Gene Symbol
      • GFAP
      • FLJ45472
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P14136 # GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
      SIZE: 432 amino acids; 49880 Da
      SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1.
      SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
      DISEASE: SwissProt: P14136 # Defects in GFAP are a cause of Alexander disease [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
      SIMILARITY: SwissProt: P14136 ## Belongs to the intermediate filament family.
      Molecular Weight50 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Materials Information
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