BackgroundInformation | Antibodiestoneuronalproteinshavebecomecriticaltoolsforidentifyingneuronsanddiscerningmorphologicalcharacteristicsincultureandcomplextissue.Whilethelabelingfromclassichistologicaltechniques,suchasGolgistaining,andmodernmolecularapproaches,suchasGFPconstructs,yieldexcellentcytoarchitecturaldetail,theseapproachesaretechnicallychallengingandimpracticalformanyneuroscienceresearchneeds.Neuron-specificantibodiesareconvenientprecisiontoolsusefulinrevealingcytoarchitecture,butarelimitedtotheproteintargetdistributionwithintheneuron,whichmaydiffergreatlyfromnucleustosomatodendriteandaxon.ToachieveascompleteamorphologicalstainingaspossIBLeacrossallpartsofneurons,Milliporepreviouslydevelopedamonoclonalantibodyblend(CatalogNo.MAB2300)thatreactsagainstkeysomatic,nuclear,dendritic,spine,andaxonalproteinsdistributedacrossthepan-neuronalarchitecture.NowMilliporehasformulatedMAB2300X,anAlexa488conjugatedversionofthepan-neuronalantibodyblendMAB2300tofacilitateimmunofluorescencestudies.EachantibodywithintheblendisconjugatedtoAlexa488thereforeenablingdetectionofanentireneuronwithonlyonemicroscopicemissionchannel.MAB2300Xhasbeenvalidatedindiversefixations,cellculture,andimmunohistochemistryprotocols,givingresearchersaconvenientandspecificqualitativeandquantitativetoolforstudyingneuronalmorphology.HighmorphologicalresolutionhasbeenachievedusingMAB2300xincludingdistraldentritesandspines. |