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Millipore/MAB1387Z | Anti-CD11b Antibody, clone M1/70.15.1, azide free/MAB1387Z/100 µg
  • Millipore/MAB1387Z | Anti-CD11b Antibody, clone M1/70.15.1, azide free/MAB1387Z/100 µg

Millipore/MAB1387Z | Anti-CD11b Antibody, clone M1/70.15.1, azide free/MAB1387Z/100 µg

價(jià)格: ¥3948.00 市場(chǎng)價(jià): 6580.00

貨號(hào): MAB1387Z
品牌: Millipore
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    • Description
      CatalogueNumberMAB1387Z
      ReplacesMAB88919
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-CD11bAntibody,cloneM1/70.15.1,azidefree
      AlternateNames
      • IntegrinalphaM
      • Mac-1a
      • MAB1387
      ProductInformation
      FormatPurified
      Control
      • Spleen,Cerebellum(Microglia)
      PresentationPurifiedratimmunoglobulininPBScontainingnopreservatives
      StorageandShippingInformation
      StorageConditionsMaintainfor2yearsat-20°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
      Applications
      ApplicationAnti-CD11bAntibody,cloneM1/70.15.1,azidefreeisanantibodyagainstCD11bforuseinFC,IF,IH&IP.
      KeyApplications
      • FlowCytometry
      • Immunofluorescence
      • Immunohistochemistry
      • Immunoprecipitation
      • AffectsFunction
      ApplicationsNotRecommended
      • Immunohistochemistry(Paraffin)
      ApplicationNotesFlowCytometry1:50-1:100

      Immunohistologyonfreshfrozenoracetonefixedtissues.

      ImmunohistochemistryonparaffinembeddedtissuesfixedinPLP,methyl-carnoy,or2%PFA;trADItional10%formalinfixationisproblematic.

      Immunoprecipitation:usearabbitanti-ratcaptureantibodyratherthanproteinGorA.

      Functional/blockingofC3bibindingtoitsreceptor.

      Optimalworkingdilutionsmustbedeterminedbyenduser.
      BIOLOGicalInformation
      ImmunogenTcellenrichedsplenocytesfromB10mice
      CloneM1/70.15.1
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostRat
      SpecificityRecognizesthemurinealphaMintegrinchain,whichexistsasaheterodimerwiththebeta2integrin(Springer,1979;Springer,1990).Theantibodystainspolymorphonuclearcells,monocytesandNKcells.Variableactivityisreportedwithtissuemacrophages.M1/70.15.1blocksiC3bbindingtoitsreceptor(Beller,1982).
      IsotypeIgG2b
      SpeciesReactivity
      • Human
      • Mouse
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodestheintegrinalphaMchain.Integrinsareheterodimericintegralmembraneproteinscomposedofanalphachainandabetachain.ThisI-domaincontainingalphaintegrincombineswiththebeta2chain(ITGB2)toformaleukocyte-specificintegrinreferredtoasmacrophagereceptor1("Mac-1"),orinactivated-C3b(iC3b)receptor3("CR3").ThealphaMbeta2integrinisimportantintheadherenceofneutrophilsandmonocytestostimulatedendothelium,andalsointhephagocytosisofcomplementcoatedparticles.SomeintegrinalphaMproteinscontainaninsertedaminoacid(Q,betweenAApositions499and500)resultingfroma3bpshiftinaspliceacceptorthatinsertsthecodonCAG;receptorswithorwithouttheextraaminoacidarefunctionallyindistinguishable
      GeneSymbol
      • ITGAM
      • MO1A
      • CD11b
      • MAC1A
      • CD11B
      • MAC-1
      • CR3A
      • MGC117044
      PurificationMethodProteinGPurified
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P11215#Integrinalpha-M/beta-2isimplicatedinvariousadhesiveinteractionsofmonocytes,macrophagesandgranulocytesaswellasinmediatingtheuptakeofcomplement-coatedparticles.ItisidenticalwithCR-3,thereceptorfortheiC3bfragmentofthethirdcomplementcomponent.ItprobablyrecognizestheR-G-DpeptideinC3b.Integrinalpha-M/beta-2isalsoareceptorforfibrinogen,factorXandICAM1.ItrecognizesP1andP2peptidesoffibrinogengammachain.
      SIZE:1152aminoacids;127179Da
      SUBUNIT:Heterodimerofanalphaandabetasubunit.Alpha-Massociateswithbeta-2.
      SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein.
      TISSUESPECIFICITY:Predominantlyexpressedinmonocytesandgranulocytes.
      DOMAIN:SwissProt:P11215TheintegrinI-domain(insert)isaVWFAdomain.IntegrinswithI-domainsdonotundergoproteasecleavage.
      SIMILARITY:Belongstotheintegrinalphachainfamily.&Contains7FG-GAPrepeats.&Contains1VWFAdomain.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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