產(chǎn)品概述:產(chǎn)品參數(shù) 外觀:可溶于 DMSO、DMF 的紅色固體 Ex/Em:490/523 nm(in MeOH) CAS 號:201860-17-5 儲(chǔ)存條件 -20℃避光保存,有效期見外包裝。 產(chǎn)品介紹 MitoSceneTM Green I 是一種線粒體綠色熒光染料,可在納摩爾水平對線粒體進(jìn)行染色標(biāo)記,該染料具有質(zhì)膜透性,且在線粒體膜上積累而呈現(xiàn)明亮的熒光。該染料在線粒體中的定位不依賴于線粒體膜電位,染色固定細(xì)胞信噪比不理想,在細(xì)胞固定、透化之后,會(huì)導(dǎo)致熒光信號減弱或丟失,所以我們推薦只用來染活細(xì)胞。注意事項(xiàng) 1. 熒光染料均存在淬滅問題,請盡量注意避光,以減緩熒光淬滅。 2. 為了您的安全和健康,請穿實(shí)驗(yàn)服并戴一次性手套操作。
說明書:UE-M4063S/M4063L MSDS:MSDS M4063 MitoSceneTM Green I
使用本產(chǎn)品的文獻(xiàn):1.Circadian clock gene NPAS2 promotes reprogramming of glucose metabolism in hepatocellular carcinoma cellsPengYuana,TaoYang,JiaoMu,JingZhao,YiYang,ZhaoyongYan,YiranHou,ChengChen,JinliangXing,HongxinZhang,JibinLiCancer Letters Volume 469, 28 January 2020, Pages 498-509參考文獻(xiàn)1.Staining of mitochondrial membranes with 10-nonyl acridine orange, MitoFluor Green, and MitoTracker Green is affected by mitochondrial membrane potential altering drugs.應(yīng)用方向:分析HL60細(xì)胞線粒體膜電位2.Efficacy of MitoTracker Green and CMXrosamine to measure changes in mitochondrial membrane potentials in living cells and tissUEs.應(yīng)用方向:對線粒體特異染色,線粒體形態(tài)和功能的研究3.MitoTracker Green labeling of mitochondrial proteins and their subseqUEnt analysis by capillary electrophoresis with laser-induced fluorescence detection.應(yīng)用方向:標(biāo)記線粒體是通過與線粒體的蛋白特異結(jié)合4.Mitotracker green is a P-glycoprotein substrate.應(yīng)用方向:染料是一種P-糖蛋白底物5.Alveolar macrophages from subjects with chronic obstructive pulmonary disease are deficient in their ability to phagocytose apoptotic airway epithelial cells.應(yīng)用方向:追蹤16HBE氣道上皮細(xì)胞的有絲分裂