Description
TheMouseBetaAmyloid1-40(Aβ40)
ELISAresearchuseonlykitistobeusedforthequantitativedeterminationofMouseandRatAβ40insamples(e.g.,cellculturesupernatants,tissuehomogenates,cerebrospinalfluid[CSF],etc.)using96-wellplatesandamicroplatereader.TheassaywillrecognizebothnaturalandsyntheticformsofMouseandRatAβ40.Theanti-mouseAβ40antibodyusedinthiskitiscapableofselectivelydetectingAβ40andnotAβ42orAβ43.
Performancecharacteristics•Sensitivity:<5>5>
•Standardcurverange:7.81–500pg/mL
•Sampletypes:cellculturesupernatants,tissuehomogenates,cerebrospinalfluid(CSF)
•Speciescross-reactivity:mouse,rat
•Samplevolume:100μL(prediluted)
•Totalassayincubationtime:4hrs
PrincipleofthemethodTheMouseAβ40kitisasolidphasesandwichEnzyme-LinkedImmunosorbentAssay(ELISA).AmonoclonalantibodytotheNH2-terminusofMouseAβhasbeencoatedontothewellsofthemicrotiterstripsprovided.Samples,includingstandardsofknownMouseAβ40content,controlspecimensandunknowns,are
Pipettedintothesewells.TheAβantigenbindstotheimmobilized(capture)antibody.Afterwashing,arabbitmonoclonalantibodyspecificfortheCOOH-terminusofthe1-40Aβsequenceisaddedtothewells.Boundrabbitantibodyisdetectedbytheuseofahorser
ADIshperoxidase-labeledanti-rabbitantibody.
Afterremovalofexcessanti-rabbitantibody,asubstratesolutionisadded,whichisacteduponbytheboundenzymetoproducecolor.TheintensityofthiscoloredproductisdirectlyproportionaltotheconcentrationofMouseAβ40presentintheoriginalspecimen.
BackgroundinformationAlzheimer’sDisease(AD)ischaracterizedbythepresenceofextracellularplaquesandintracellularneurofibrillarytangles(NFTs)inthebrain.Themajorproteincomponentoftheseplaquesisbetaamyloidpeptide(Aβ),a40to43aminoacidpeptidecleavedfromamyloidprecursorproteinbyβ-secretase(e.g.,BACE)andaputativeγ(gamma)secretase.Increasedreleaseofthe‘longerforms’ofAβpeptide,Aβ42orAβ43,whichhaveagreatertendencytoaggregatethanAβ40,occursinindividualsexpressingcertaingeneticmutations,expressingcertainApoEalleles,ormayinvolveother,stillundiscovered,factors.ManyresearcherstheorizethatitisthisincreasedreleaseofAβ42/Aβ43whichleadstotheabnormaldepositionofAβandtheassociatedneurotoxicityinthebrainsofaffectedindividuals.
EachMouseAβ40ELISAkitisvalidatedforsensitivity,specificity,precision,andlot-to-lotconsistency.Seeproductinsertformoreinformationonvalidation.
RelatedlinksLearnmoreaboutELISAkits
Learnmoreaboutotherimmunoassays
*PleaserefertotheELISAprotocolforspecificreferencecitations.
ForResearchUseOnly.Notforuseindiagnosticprocedures.