Antibody reactive against mammalian transfected lysate.
Storage Buffer:
In 1x PBS, pH 7.4
Storage Instruction:
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
MSDS:
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Interspecies Antigen Sequence:
Mouse (90); Rat (91)
Datasheet:
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Applications
Western Blot (Tissue lysate)
ACF MaxPab polyclonal antibody. Western Blot analysis of ACF expression in human placenta.
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Western Blot (Cell lysate)
A1CF MaxPab polyclonal antibody. Western Blot analysis of A1CF expression in HeLa.
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Western Blot (Transfected lysate)
Western Blot analysis of A1CF expression in transfected 293T cell line (H00029974-T02) by A1CF MaxPab polyclonal antibody.Lane 1: ACF transfected lysate(65.34 KDa).Lane 2: Non-transfected lysate.
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Immunofluorescence
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Immunofluorescence of purified MaxPab antibody to A1CF on HeLa cell. [antibody concentration 10 ug/ml]
Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events. Alternative splicing occurs at this locus and three full-length transcript variants, encoding three distinct isoforms, have been described. Additional splicing has been observed but the full-length nature of these variants has not been determined. [provided by RefSeq