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Abnova/NME1 monoclonal antibody (M02), clone 1D7/1kit/H00004830-M02
  • Abnova/NME1 monoclonal antibody (M02), clone 1D7/1kit/H00004830-M02

Abnova/NME1 monoclonal antibody (M02), clone 1D7/1kit/H00004830-M02

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貨號(hào): H00004830-M02
品牌: Abnova
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  • 詳情
  • 使用說(shuō)明
  • 常見(jiàn)問(wèn)題
      • Specification
      • Gene Info
      • Pathway
      • Disease
      • Related Products
      • List All
      • Specification
      • Product Description:
      • Mouse monoclonal antibody raised against a partial recombinant NME1.
      • Immunogen:
      • NME1 (NP_000260, 43 a.a. ~ 152 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
      • Sequence:
      • ASEDLLKEHYVDLKDRPFFAGLVKYMHSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVESAEKEIGLWFHPEELVDYTSCAQNWIYE
      • Host:
      • Mouse
      • Reactivity:
      • Human
      • Isotype:
      • IgG1 Kappa
      • Quality Control Testing:
      • Antibody Reactive Against Recombinant Protein.QC Testing of H00004830-M02Western Blot detection against Immunogen (37.84 KDa) .
      • Storage Buffer:
      • In 1x PBS, pH 7.4
      • Storage Instruction:
      • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
      • MSDS:
      • PDF DownloadDownload
      • Interspecies Antigen Sequence:
      • Mouse (94%); Rat (95%)
      • Datasheet:
      • PDF DownloadDownload
      • Publication Reference
      • 1.
      • A transcriptome-based protein network that identifies new therapeutic targets in colorectal cancer.Durand S, Trillet K, Uguen A, Saint-Pierre A, Le Jossic-Corcos C, Corcos L.BMC Genomics. 2017 Sep 30;18(1):758.
      • 2.
      • Identification of Antigenic Proteins Associated with Trichloroethylene-Induced Autoimmune Disease by Serological Proteome Analysis.Liu J, Xing X, Huang H, Jiang Y, He H, Xu X, Yuan J, Zhou L, Yang L, Zhuang Z.Toxicol Appl Pharmacol. 2009 Nov 1;240(3):393-400. Epub 2009 Aug 6.
      • Applications
      • Western Blot (Cell lysate)
      • Western Blot (Cell lysate)
      • NME1 monoclonal antibody (M02), clone 1D7. Western Blot analysis of NME1 expression in HeLa(Cat # L013V1 ).
      • PDF DownloadProtocol Download
      • Western Blot (Cell lysate)
      • Western Blot (Cell lysate)
      • NME1 monoclonal antibody (M02), clone 1D7. Western Blot analysis of NME1 expression in different cell lines.
      • PDF DownloadProtocol Download
      • Western Blot (Transfected lysate)
      • Western Blot (Transfected lysate)
      • Western Blot analysis of NME1 expression in transfected 293T cell line by NME1 monoclonal antibody (M02), clone 1D7.Lane 1: NME1 transfected lysate(19.7 KDa).Lane 2: Non-transfected lysate.
      • PDF DownloadProtocol Download
      • Western Blot (Recombinant protein)
      • PDF DownloadProtocol Download
      • Immunofluorescence
      • Immunofluorescenceenlargeenlarge this image
      • Immunofluorescence of monoclonal antibody to NME1 on HeLa cell. [antibody concentration 10 ug/ml]
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)enlargeenlarge this image
      • Immunoperoxidase of monoclonal antibody to NME1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
      • PDF DownloadProtocol Download
      • Immunoprecipitation
      • Immunoprecipitation
      • Immunoprecipitation of NME1 transfected lysate using anti-NME1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with NME1 MaxPab rabbit polyclonal antibody.
      • PDF DownloadProtocol Download
      • Sandwich ELISA (Recombinant protein)
      • Sandwich ELISA (Recombinant protein)
      • Detection limit for recombinant GST tagged NME1 is approximately 0.1ng/ml as a capture antibody.
      • PDF DownloadProtocol Download
      • ELISA
      • Application Image
      • Western Blot (Cell lysate)
      • Western Blot (Cell lysate)
      • enlarge
      • Western Blot (Cell lysate)
      • Western Blot (Cell lysate)
      • enlarge
      • Western Blot (Transfected lysate)
      • Western Blot (Transfected lysate)
      • enlarge
      • Western Blot (Recombinant protein)
      • Immunofluorescence
      • Immunofluorescence
      • enlarge
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      • enlarge
      • Immunoprecipitation
      • Immunoprecipitation
      • enlarge
      • Sandwich ELISA (Recombinant protein)
      • Sandwich ELISA (Recombinant protein)
      • enlarge
      • ELISA
      • Gene Information
      • Entrez GeneID:
      • 4830
      • GeneBank Accession#:
      • NM_000269
      • Protein Accession#:
      • NP_000260
      • Gene Name:
      • NME1
      • Gene Alias:
      • AWD,GAAD,NB,NBS,NDPK-A,NDPKA,NM23,NM23-H1
      • Gene Description:
      • non-metastatic cells 1, protein (NM23A) expressed in
      • Omim ID:
      • 156490, 256700
      • Gene Ontology:
      • Hyperlink
      • Gene Summary:
      • This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of "A" (encoded by this gene) and "B" (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq
      • Other Designations:
      • NDP kinase A,OTTHUMP00000174772
      • Gene Pathway
      • Metabolic pathways
      • Purine metabolism
      • Pyrimidine metabolism
      • Related Disease
      • Breast cancer
      • Breast Neoplasms
      • Carcinoma, Non-Small-Cell Lung
      • Endometrial Neoplasms
      • Genetic Predisposition to Disease
      • Lung Neoplasms
      • Neoplasm Metastasis
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