Properties:
| Related Categories |
Analytical/Chromatography,
Antibodies,
Antibody Purification and Characterization,
HiTrap & PD-10 Cartridges,
Protein A, G and L Resins,
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shelf life
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Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
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packaging
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pack of 1 mL
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mfr. no.
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GE Healthcare, 29-0491-04
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availability
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not available in North America
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parameter
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<4 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
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42 psi
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bed size
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7 mm × 25 mm
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bed volume
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1 mL
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column I.D.
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7 mm
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matrix
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highly cross-linked agarose
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avg. part. size
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85 μm (d??v is the median particle size of the cumulative volume distribution)
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cleaning
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2 - 13.7 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
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working range
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3 - 12 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
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capacity
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≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)(Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.)
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suitability
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suitable for bioprocess medium
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storage temp.
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2-8°C
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Description:
Legal Information
Free MabSelect SuRe Ligand
"MabSelect SuRe Ligand Restricted License" and "Cys-rProtein A Ligand Restricted License" are protected by the following patents and equivalent patents and patent applications in other countries: US 6,399,750 and EP 1123389. A free, non-transferable limited license to use this product for internal analytical purposes only accompanies the purchase of the product from a GE Healthcare company and its licensed distributors. Any other use will require a separate license from a GE Healthcare company.
HiTrap is a registered trademark of GE Healthcare
MabSelect SuRe is a trademark of GE Healthcare
SuRe is a trademark of GE Healthcare
Features and Benefits
• Withstands rigorous CIP and sanitization procedures with 0.1 to 0.5 M NaOH.
• Novel ligand design gives enhanced protease resistance resulting in lower ligand leakage.
• Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
• Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
• Prepacked HiTrap columns for process development, screening of purification conditions, and small-scale purification of Mabs.
General description
HiTrap MabSelect SuRe columns are prepacked with MabSelect SuRe, a BioProcess medium for purification of monoclonal antibodies from large sample volumes. The novel alkali-tolerant recombinant Protein A ligand allows cleaning-in-place (CIP).
Safety:
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