Properties:
Related Categories |
Analytical/Chromatography,
GE Life Sciences Gel Filtration Columns,
Gel Filtration Chromatography,
HPLC,
HPLC & FPLC Columns for Biopolymer Separations,
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material
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polypropylene (PP)
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shelf life
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Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
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mol wt
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mol wt 20000-8000000 Da (globular proteins)
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packaging
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pkg of 1 ea
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mfr. no.
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GE Healthcare, 28-9356-04
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parameter
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<0.5 mL/min flow rate
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0.15 Mpa (22 psi)
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bed volume
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120 mL
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column L × I.D.
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60 cm × 16 mm
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sample volume
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<5 mL
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matrix
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dextran-acrylamide particle platform
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allyl dextran and N, N’-methylenebisacrylamide
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matrix active group
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dextran-acrylamide phase
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avg. part. size
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50 μm
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cleaning
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2 - 13
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working range
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3 - 11
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mode of chromatography
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Size exclusion (SEC)
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Description:
Analysis Note
To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.
Application
Sephacryl® S-400 is optimized for separating polysaccarides and other macromolecules with extended structures e.g. proteoglycans and liposomes.
Features and Benefits
• Gel filtration columns for low to medium pressure AKTA systems.
• The HiPrep™ Sephacryl® series offer reproducible separations over a wide molecular weight range.
• Convenient, prepacked HiPrep™ Sephacryl® columns speed up protein purification.
• Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/ bisacrylamide matrix.
General description
HiPrep™ 16/60 Sephacryl® S-400 HR is a prepacked gel filtration column for preparative separation of biomolecules in the molecular weight range of 20 000 to 8 000 000 with sample volumes up to 5 mL.
Recommended products
Discover LiChropur reagents ideal for HPLC or LC-MS analysis
Suitability
All commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40oC for 1 week), 0.5 M NaOH (only for cleaning-in-place)
gel filtration columns for low to medium pressure ?KTA systems+AE14
Legal Information
HiPrep is a trademark of GE Healthcare
Sephacryl is a registered trademark of GE Healthcare
Safety:
RIDADR
NONH for all modes of transport