Properties:
Related Categories |
Analytical/Chromatography,
GE Healthcare Life Sciences Protein Sample Preparation Formats,
HiTrap & PD-10 Cartridges,
HiTrap System/syringe,
Molecular Biology,
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description
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Ion Exchanger Type (value)
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shelf life
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Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
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packaging
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pack of 5 × 5 mL
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mfr. no.
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GE Healthcare, 17-5163-01
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parameter
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<20 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
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42 psi
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bed size
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16 mm × 25 mm
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bed volume
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5 mL
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column I.D.
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16 mm
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matrix
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4% cross-linked agarose
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particle size
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45-165 μm
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average diameter
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90 μm
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cleaning
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2 - 14
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working range
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3 - 13
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suitability
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suitable for bioprocess medium
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Description:
Analysis Note
www.gelifesciences.com.
Application
Q, SP, DEAE, and CM Sepharose® Fast FLow are based on a robust, 6% highly cross-linked beaded Agarose matrix with good flow properties and high loading capacities. ANX Sepharose® 4 Fast FLow (high sub) is based on 4% highly cross-linked beaded Agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass Proteins.
The active end of the charged group is the same for DEAE Sepharose Fast FLow and ANX Sepharose® Fast FLow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose Fast FLow has a diethylaminoethyl-group bound to the Agarose whilst ANX Sepharose® 4 Fast FLow (high sub) has a diethylaminopropyl group attached.
Features and Benefits
• Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
• The industry standard for ion exchange chromatography.
• High fLow rates and good scale-up potential.
• Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
• Predictable scale-up
General description
HiTrap® ANX (high sub) FF is prepacked with ANX (high sub) Sepharose® Fast Flow, and is a weak anion exchanger for small scale purification of higher molecular weight protein as well as screening of binding and elutions conditions.
Legal Information
HiTrap is a registered trademark of GE Healthcare
Sepharose is a registered trademark of GE Healthcare
Safety:
Symbol
GHS02
Precautionary statements
P210-P370 + P378
RIDADR
UN1170 - class 3 - PG 3 - Ethanol, solution