Properties:
RelatedCategories | Analytical/Chromatography,GEDesaltingColumns,GelFiltrationChromatography,HPLC,HPLC&FPLCColumnsforBiopolymerSeparations, |
shelflife | Pleasebeawarethisproductmaybeshipped90daysbeforetheexpirationdate.Formoreinformationonthebatchspecificexpirationdate,pleasecontacttechnicalservice. |
feature | wettedpart:no |
packaging | packof30 pieces |
mfr.no. | GEHealthcare,17-0851-01 |
storagecondition | drymedia |
bedsize | 14.5 mm×50 mm |
bedvolume | 8.3 mL |
columnI.D. | 15 mm |
matrix | cross-linkeddextran |
particlesize | >50 μm(dry) |
| 85-260 μm |
cleaning | 2-13 |
workingrange | 2-13 |
suitABIlity | suitableforbioprocessmedium |
Description:
FeaturesandBenefits
• Fast,affordable,high-recoverydesaltingandbufferexchange,aswellasremovaloflow-molecularweightcompoundspackedwithSephadex®G-25Medium.
• FlexIBLeprotocolsallowrunsbygravityfloworcentrifugation.
• Easytouseandequilibrate;applysample;elute.
• Preparationofthesamplesintherangeof1.0to2.5mLbygravityflowand1.75to2.5mLwithcentrifugation.
• Desaltmultiplesamplesinparallelwithadesaltingcapacity>90%.
• Recoveryintherange70to>95%.
Generaldescription
DisposablePD-10DesaltingColumnsofferaninexpensiveandconvenientalternativetolengthyandtroublesomedialysisprocedures.Inminutes,BIOLOGicalsamplesaredesaltedandsmallmolecularweightsolutesareremovedorexchangedwithexcellentrecoveriesofmacromolecules.
TheinstructionsforDisposablePD-10DesaltingColumnshavebeenupdatedwithtwonewapplicationprotocols:gravityorcentrifugation,toincreasetheflexibilityoftheproduct.Thegravityprotocolallowsasimplecleanupofsampleswithoneorseveralcolumnsinparallel,withouttheneedforapurificationsystem.Thespinprotocolenablesrunsinparallelinastandardcentrifugewithminimaldilutionoftheelutedsample.
Fouradaptersareincludedineachproductpacktoenableeasycentrifugation.TogetherwithLaBMAtePD-10BufferReservoir,washandequilibrationbuffercanbeappliedinonestepwiththegravityflowprotocol.EmptyPD-10columnsarerecommendedforusewithbulkmediaforaffordableandversatilesamplepreparation.
LegalInformation
SephadexisaregisteredtrademarkofGEHealthcare
Safety:
Precautionarystatements
P261-P272-P280-P302+P352-P333+P313-P501