Properties:
RelatedCategories | Analytical/Chromatography,GEHealthcareLifeSciencesProteinSamplePreparationFormats,HiTrap&PD-10Cartridges,HiTrapSystem/syringe,MolecularBIOLOGy, |
shelflife | Pleasebeawarethisproductmaybeshipped90daysbeforetheexpirationdate.Formoreinformationonthebatchspecificexpirationdate,pleasecontacttechnicalservice. |
packaging | packof5×5 mL |
mfr.no. | GEHealthcare,17-0407-03 |
parameter | <20mL/minflowrate(Thepressureoverthepackedbedvariesdependingonarangeofparameterssuchasthecharacteristicsofthechromatographymediumandthecolumntubingused.) |
| 42psi |
bedsize | 16 mm×25 mm |
bedvolume | 5 mL |
columnI.D. | 16 mm |
matrix | highlycross-linked6%agarose |
avg.part.size | 34 μm |
cleaning | 5-10 |
workingrange | 5-10 |
Description:
AnalysisNote
ToviewtheCertificateofAnalysisforthisproduct,pleasevisitwww.geLifesciences.com.
FeaturesandBenefits
• Heparinbindstoawiderangeofbiomoleculesviabothaffinityandionexchangemechanisms.
• ImmobilizedheparinisusedforpurificationofantithrombinIII,coagulationfactors,andotherplasmaproteins.
• Simpleoperationwithasyringe,apumporachromatographysystemsuchasAKTAdesign
• StableoverpH5to10andtoleratesallcommonlyusedaqueousbuffers.
Generaldescription
HiTrap®HeparinHPcolumnsareprepackedwithHeparinSepharose®HighPerformanceforhigh-resolutionpurificationofproteinswithaffnityforheparinsuchasDNA-bindingproteins,coagulationfactors,lipoproteins,andproteinsynthesisfactors.
Immobilizedheparinhastwomainmodesofinteractionwithproteins.Itcanoperateasanaffinityligandwithaffinityforawiderangeofbiomolecules,includingcoagulationfactorsandotherplasmaproteins,lipoproteins,proteinsynthesisfactors,enzymesthatactonnucleicacids,andsteroidreceptors.Heparinalsofunctionsasahighcapacitycationexchanger,duetoitsanionicsulfategroups.GrADIentelutionwithsaltismostcommonlyusedinbothcases.
HepariniscoupledtotheSepharose®HighPerformancebasematrixviatheN-hydroxysuccinamidecouplingmethod,togivehighcapacity,performance,andlowleakagelevels.HiTrap®HeparinHPisdeliveredin0.2MNaClwith20%ethanolasbacteriostaticagent.StoreHiTrap®HeparinHPcolumnsequilibratedwith25mLof20%ethanolorabuffercontainingabacteriostaticagent.EquilibrationiseasilyperformedwithasyringeandthesuppliedLueradapter.Sealthecolumnwiththesuppliedstopperanddomednut.Therecommendedstoragetemperatureis4°Cto30°C.
LegalInformation
HiTrapisaregisteredtrademarkofGEHealthcare
SepharoseisaregisteredtrademarkofGEHealthcare
Safety:
Symbol
GHS02
Precautionarystatements
P210-P370+P378
RIDADR
UN1170-class3-PG3-Ethanol,solution