Biomolecules can be immobilized by binding them to a support under conditions that will then determine the characteristics of the complex. Immobilization is a technique that binds a ligand (enzyme, antibody, affinity proteins like Protein A or Protein G) to a support, giving high stability and making it easier to reuse the immobilized molecule.
Glyoxal beads allow a covalent binding of agarose to lysine aminogroups (similar orientation to CNBr). Glyoxal agarose beads are ready touse and require no activation. The resin has a high amount of aldehydesgroups (40-60 μmoles Glyoxal / ml gel) providing a very good option toconjugate affinity ligands improving the stability of the ligand.
The choice of Glyoxal or Aminoethyl will depend on the biomolecule to be immobilized, the accessibility of the reactive groups, and the direction/ orientation required for the binding to the support.
High & Very High Density Resin vs. Low & Very Low Density Resin? High/Very High binding capacity ? Good binding capacity? High immobilized enzyme stability? Immobilized enzyme stability? Possibility of multiple binding points? Minimum distortion of immobilized enzyme
Glyoxal 6% agarose beads are suitable for use with FPLC, but with pressures no more than 20 kPa. Too much pressure or too fast of a flow rate will result in diminished performance.
Storage/Handling: Store at 4°C. Do not freeze.
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