Labeled FISH probes for identification of gene translocation using Fluorescent In Situ Hybridization Technique. (Technology)
Form:
Liquid
Quality Control Testing:
Representative images of normal human cell (lymphocyte) stain with the dual color FISH probe. The left image is chromosomes at metaphase, and the right image is an interphase nucleus.
Supplied Product:
DAPI Counterstain (1500 ng/mL ) 125 uL for each 100 uL FISH Probe
Storage Instruction:
Store at 4°C in the dark.
Note:
Hybridization position of the probes on the chromosome:
Probe 1:Size:Fluorophore:Location:
NCOA2Approximately 1300kbTexas Red8q13.3
Probe 2:Size:Fluorophore:Location:
HEY1Approximately 1250kbFITC8q21.13
Probe Gap:
The gap between two probes is approximately 8,200 kb.
Origin:
Human
Source:
Genomic DNA
Notice:
We strongly recommend the customer to use FFPE FISH PreTreatment Kit 1 (Catalog #: KA2375 or KA2691) for the pretreatment of Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections.
Regulation Status:
For research use only (RUO)
Datasheet:
Download
Applications
Fluorescent In Situ Hybridization (Cell)
Protocol Download
Fluorescent In Situ Hybridization (Formalin/PFA-fixed paraffin-embedded sections)
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Huma lung, adenosquamous cell carcinoma (FFPE) stained with HEY1/NCOA2 DY Translocation FISH Probe. Human lung cancer showed no HEY/NCOA2 DY Translocation.
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Fluorescent In Situ Hybridization (Formalin/PFA-fixed paraffin-embedded sections)
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Human hemangiopericytoma (FFPE) stained with HEY1/NCOA2 DY Translocation FISH Probe. Human hemangiopericytoma showed no HEY1/NCOA2 DY translocation.
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Fluorescent In Situ Hybridization (Formalin/PFA-fixed paraffin-embedded sections)
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Human mesenchymal chondrosarcoma (FFPE) stained with HEY1/NCOA2 DY Translocation FISH Probe. Human mesenchymal chondrosarcoma showed no HEY1/NCOA2 DY translocation.
Protocol Download
Application Image
Fluorescent In Situ Hybridization (Cell)
Fluorescent In Situ Hybridization (Formalin/PFA-fixed paraffin-embedded sections)
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Fluorescent In Situ Hybridization (Formalin/PFA-fixed paraffin-embedded sections)
enlarge
Fluorescent In Situ Hybridization (Formalin/PFA-fixed paraffin-embedded sections)
enlarge
NCOA2
HEY1
Gene Information
Entrez GeneID:
10499
Gene Name:
NCOA2
Gene Alias:
GRIP1,KAT13C,MGC138808,NCoA-2,TIF2,bHLHe75
Gene Description:
nuclear receptor coactivator 2
Omim ID:
601993
Gene Ontology:
Hyperlink
Other Designations:
glucocorticoid receptor-interacting protein-1
Gene Information
Entrez GeneID:
23462
Gene Name:
HEY1
Gene Alias:
BHLHb31,CHF2,HERP2,HESR1,HRT-1,MGC1274,OAF1
Gene Description:
hairy/enhancer-of-split related with YRPW motif 1
Omim ID:
602953
Gene Ontology:
Hyperlink
Gene Summary:
This gene encodes a nuclear protein belonging to the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcriptional repressors. Expression of this gene is induced by the Notch and c-Jun signal transduction pathways. Two similar and redundant genes in mouse are required for embryonic cardiovascular development, and are also implicated in neurogenesis and somitogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations:
HES-related repressor protein 2,basic helix-loop-helix protein OAF1,cardiovascular helix-loop-helix factor 2,hairy-related transcription factor 1