Synonym:TriglycerideandFreeGlycerolKitsandReagentsProperties:
RelatedCategories | AssayKitsForStudyingFattyAcidMetabolism,BiochemicalsandReagents,BloodandUrineChemistryAssayKits,CancerMetabolism,CancerResearch, |
usage | 1 kitsufficientfor1000 reactions |
storagetemp. | 2-8°C |
Description:
Linkage
Inadditiontokits,theindividualreagentsandglycerolstandardareavailableseparatelywhenfewerreactionsareneeded.
Application
TheFreeGlycerolDeterminationKithasbeenusedtodeterminethelevelofglycerolreleasedintothemediumfrommammaliancellsgrowninvitro.
TheFreeGlycerolDeterminationKitmeasuresfree,endogenousglycerolusingcoupledenzymereactionsanddoesnotincludeinitiallipasehydrolysis.Glycerolisphosphorylatedbyadenosine-5′-triphosphate(ATP)formingglycerol-1-phosphate(G-1-P)andadenosine-5′-diphosphate(ADP)inthereactioncatalyzedbyglycerolkinase(GK).G-1-Pisthenoxidizedbyglycerolphosphateoxidase(GPO)todihydroxyacetonephosphate(DAP)andhydrogenperoxide(H2O2).PeroxidasecatalyzesthecouplingofH2O2with4-aminoantipyrine(4-AAP)andsodiumN-ethyl-N-(3-sulfopropyl)m-anisidine(ESPA)toproduceaquinoneiminedyethatshowsanabsorbancemaximumat540nm.Theincreaseinabsorbanceat540nmisdirectlyproportionaltothefreeglycerolconcentrationofthesample.
Thetriglycerideandfreeglycerolkitsareforthequantitativedeterminationofglycerol,totaltriglyceridesorfreetriglycerides.
Safety:
Symbol
GHS07,GHS09
Hazardstatements
H319-H411
Precautionarystatements
P273-P305+P351+P338