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Abnova/CAMK2A(phospho T286) & CAMK2A Protein Phosphorylation Antibody Pair/1kit/DP0227
  • Abnova/CAMK2A(phospho T286) & CAMK2A Protein Phosphorylation Antibody Pair/1kit/DP0227

Abnova/CAMK2A(phospho T286) & CAMK2A Protein Phosphorylation Antibody Pair/1kit/DP0227

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貨號: DP0227
品牌: Abnova
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  • 詳情
  • 使用說明
  • 常見問題
      • Specification
      • Gene Info
      • Interactome
      • Pathway
      • Disease
      • Related Products
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      • Specification
      • Product Description:
      • This protein phosphorylation antibody pair set comes with two antibodies, one against the CAMK2A protein, and the other against the specific T286 phosphorylated site of CAMK2A for use in in situ Proximity Ligation Assay. See Publication Reference below.DuoLink
      • Reactivity:
      • Human
      • Quality Control Testing:
      • Dual recognition immunofluorescence result.QC Testing of DP0227Representative image of Proximity Ligation Assay of protein phosphorylation. HeLa cells were stained with dual recognition antibody pair set, rabbit polyclonal antibody 1:1200 and mouse monoclonal antibody 1:50. Each red dot represents one single phosphorylated protein. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
      • Supplied Product:
      • Antibody pair set content: 1. Phospho-CAMK2A T286 rabbit polyclonal antibody (20 ul) In PBS, 150 mM NaCl, pH 7.4 (0.02% sodium azide, 50% glycerol) 2. CAMK2A mouse monoclonal antibody (40 ug) In 1x PBS, pH 7.2 *Reagents are sufficient for at least 30-50 assays using recommended protocols.
      • Storage Instruction:
      • Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
      • Datasheet:
      • PDF DownloadDownload
      • Publication Reference
      • 1.
      • In situ detection of phosphorylated platelet-derived growth factor receptor beta using a generalized proximity ligation method.Jarvius M, Paulsson J, Weibrecht I, Leuchowius KJ, Andersson AC, Wahlby C, Gullberg M,Botling J,Sjoblom T, Markova B, Ostman A, Landegren U, Soderberg O.Mol Cell Proteomics. 2007 Sep;6(9):1500-9. Epub 2007 Jun 12.
      • 2.
      • Direct observation of individual endogenous protein complexes in situ by proximity ligation.Soderberg O, Gullberg M, Jarvius M , Ridderstrale K, Leuchowius KJ, Jarvius J, Wester K, Hydbring P, Bahram F, Larsson LG, and Landegren U. Nat Methods. 2006 Dec;3(12):995-1000. Epub 2006 Oct 29.
      • 3.
      • Cytokine detection by antibody-based proximity ligation.Gullberg M, Gustafsdottir SM, Schallmeiner E, Jarvius J, Bjarnegard M, Betsholtz C, Landegren U, andFredriksson S. Proc Natl Acad Sci U S A. 2004 Jun 1;101(22):8420-4. Epub 2004 May 21.
      • 4.
      • Protein detection using proximity-dependent DNA ligation assays.Fredriksson S, GullbergM, Jarvius J, Olsson C, Pietras K, Gustafsdottir SM, Ostman A, and Landegren U. Nat Biotechnol. 2002 May;20(5):473-7.
      • 5.
      • Highly specific detection of phosphorylated proteins by DuolinkMats Gullberg and Ann-Catrin Andersson Nature Methods 6. 2009
      • Applications
      • In situ Proximity Ligation Assay (Cell)
      • Application Image
      • In situ Proximity Ligation Assay (Cell)
      • Gene Information
      • Entrez GeneID:
      • 815
      • Gene Name:
      • CAMK2A
      • Gene Alias:
      • CAMKA,KIAA0968
      • Gene Description:
      • calcium/calmodulin-dependent protein kinase II alpha
      • Omim ID:
      • 114078
      • Gene Ontology:
      • Hyperlink
      • Gene Summary:
      • The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq
      • Other Designations:
      • CaM kinase II alpha subunit,CaM-kinase II alpha chain,CaMK-II alpha subunit,CaMKIINalpha,OTTHUMP00000165787,OTTHUMP00000165788,calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha,calcium/calmodulin-dependent protein kinase II alpha-B subunit
      • Interactome
      • Interactome
      • Gene Pathway
      • Calcium signaling pathway
      • ErbB signaling pathway
      • Glioma
      • GnRH signaling pathway
      • Long-term potentiation
      • Melanogenesis
      • Neurotrophin signaling pathway
      • Olfactory transduction
      • Wnt signaling pathway
      • Related Disease
      • Bipolar Disorder
      • Cognition
      • Genetic Predisposition to Disease
      • Schizophrenia
      • Schizophrenia
      • Schizophrenic Psychology
      • Tobacco Use Disorder
      • Weight Gain
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