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Abnova/ATM(phospho S1981) & ATM Protein Phosphorylation Antibody Pair/1kit/DP0076
  • Abnova/ATM(phospho S1981) & ATM Protein Phosphorylation Antibody Pair/1kit/DP0076

Abnova/ATM(phospho S1981) & ATM Protein Phosphorylation Antibody Pair/1kit/DP0076

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貨號: DP0076
品牌: Abnova
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  • 詳情
  • 使用說明
  • 常見問題
      • Specification
      • Gene Info
      • Pathway
      • Disease
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      • Specification
      • Product Description:
      • This protein phosphorylation antibody pair set comes with two antibodies, one against the ATM protein, and the other against the specific S1981 phosphorylated site of ATM for use in in situ Proximity Ligation Assay. See Publication Reference below.DuoLink
      • Reactivity:
      • Human
      • Quality Control Testing:
      • Dual recognition immunofluorescence result.QC Testing of DP0076Representative image of Proximity Ligation Assay of protein phosphorylation. HeLa cells were stained with dual recognition antibody pair set, rabbit polyclonal antibody 1:1200 and mouse monoclonal antibody 1:50. Each red dot represents one single phosphorylated protein. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
      • Supplied Product:
      • Antibody pair set content: 1. Phospho-ATM S1981 rabbit polyclonal antibody (20 ul) In PBS (0.09% (w/v) sodium azide) 2. ATM mouse monoclonal antibody (40 ug) In 1x PBS, pH 7.2 *Reagents are sufficient for at least 30-50 assays using recommended protocols.
      • Storage Instruction:
      • Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
      • Datasheet:
      • PDF DownloadDownload
      • Publication Reference
      • 1.
      • In situ detection of phosphorylated platelet-derived growth factor receptor beta using a generalized proximity ligation method.Jarvius M, Paulsson J, Weibrecht I, Leuchowius KJ, Andersson AC, Wahlby C, Gullberg M,Botling J,Sjoblom T, Markova B, Ostman A, Landegren U, Soderberg O.Mol Cell Proteomics. 2007 Sep;6(9):1500-9. Epub 2007 Jun 12.
      • 2.
      • Direct observation of individual endogenous protein complexes in situ by proximity ligation.Soderberg O, Gullberg M, Jarvius M , Ridderstrale K, Leuchowius KJ, Jarvius J, Wester K, Hydbring P, Bahram F, Larsson LG, and Landegren U. Nat Methods. 2006 Dec;3(12):995-1000. Epub 2006 Oct 29.
      • 3.
      • Cytokine detection by antibody-based proximity ligation.Gullberg M, Gustafsdottir SM, Schallmeiner E, Jarvius J, Bjarnegard M, Betsholtz C, Landegren U, andFredriksson S. Proc Natl Acad Sci U S A. 2004 Jun 1;101(22):8420-4. Epub 2004 May 21.
      • 4.
      • Protein detection using proximity-dependent DNA ligation assays.Fredriksson S, GullbergM, Jarvius J, Olsson C, Pietras K, Gustafsdottir SM, Ostman A, and Landegren U. Nat Biotechnol. 2002 May;20(5):473-7.
      • 5.
      • Highly specific detection of phosphorylated proteins by DuolinkMats Gullberg and Ann-Catrin Andersson Nature Methods 6. 2009
      • Applications
      • In situ Proximity Ligation Assay (Cell)
      • Application Image
      • In situ Proximity Ligation Assay (Cell)
      • Gene Information
      • Entrez GeneID:
      • 472
      • Gene Name:
      • ATM
      • Gene Alias:
      • AT1,ATA,ATC,ATD,ATDC,ATE,DKFZp781A0353,MGC74674,TEL1,TELO1
      • Gene Description:
      • ataxia telangiectasia mutated
      • Omim ID:
      • 114480, 208900, 607585
      • Gene Ontology:
      • Hyperlink
      • Gene Summary:
      • The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
      • Other Designations:
      • AT mutated,TEL1, telomere maintenance 1, homolog,ataxia telangiectasia mutated (includes complementation groups A, C and D),ataxia telangiectasia mutated protein,human phosphatidylinositol 3-kinase homolog,serine-protein kinase ATM
      • Gene Pathway
      • Apoptosis
      • Cell cycle
      • p53 signaling pathway
      • Related Disease
      • Acute Disease
      • Adenocarcinoma
      • Ataxia Telangiectasia
      • Ataxia telangiectasia
      • Atherosclerosis
      • Brain Neoplasms
      • Breast cancer
      • Breast Neoplasms
      • Carcinoma
      • Carcinoma, Ductal, Breast
      • Carcinoma, Lobular
      • Carcinoma, Non-Small-Cell Lung
      • Carcinoma, Pancreatic Ductal
      • Carcinoma, Squamous Cell
      • Cardiovascular Diseases
      • Chromosome Aberrations
      • Cocarcinogenesis
      • Colorectal Neoplasms
      • Colorectal Neoplasms, Hereditary Nonpolyposis
          • ... see more
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