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Abnova/AKT1 & BAD Protein Protein Interaction Antibody Pair/1kit/DI0242
  • Abnova/AKT1 & BAD Protein Protein Interaction Antibody Pair/1kit/DI0242

Abnova/AKT1 & BAD Protein Protein Interaction Antibody Pair/1kit/DI0242

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貨號: DI0242
品牌: Abnova
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  • 詳情
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      • Specification
      • Gene Info
      • Interactome
      • Related Products
      • List All
      • Specification
      • Product Description:
      • This protein protein interaction antibody pair set comes with two antibodies to detect the protein-protein interaction, one against the AKT1 protein, and the other against the BAD protein for use in in situ Proximity Ligation Assay. See Publication Reference below.DuoLink
      • Reactivity:
      • Human
      • Quality Control Testing:
      • Protein protein interaction immunofluorescence result.QC Testing of DI0242Representative image of Proximity Ligation Analysis of protein-protein interactions between AKT1 and BAD. HeLa cells were stained with anti-AKT1 rabbit purified polyclonal antibody 1:100 and anti-BAD mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
      • Supplied Product:
      • Antibody pair set content: 1. AKT1 rabbit purified polyclonal antibody (100 ug) 2. BAD mouse monoclonal antibody (40 ug) *Reagents are sufficient for at least 30-50 assays using recommended protocols.
      • Storage Instruction:
      • Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
      • MSDS:
      • PDF DownloadDownload
      • Datasheet:
      • PDF DownloadDownload
      • Publication Reference
      • 1.
      • An analysis of protein-protein interactions in cross-talk pathways reveals CRKL as a novel prognostic marker in hepatocellular carcinoma.Liu CH, Chen TC, Chau GY, Jan YH, Chen CH, Hsu CN, Lin KT, Juang YL, Lu PJ, Cheng HC, Chen MH, Chang CF, Ting YS, Kao CY, Hsiao M, Huang CY. Mol Cell Proteomics. 2013 Feb 8. [Epub ahead of print]
      • Applications
      • In situ Proximity Ligation Assay (Cell)
      • <em>In situ</em> Proximity Ligation Assay (Cell)
      • Representative image of Proximity Ligation Assay of protein-protein interactions between AKT1 and BAD. PC-3 cells were stained with anti-AKT1 rabbit purified polyclonal antibody 1:100 and anti-BAD mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
      • In situ Proximity Ligation Assay (Cell)
      • <em>In situ</em> Proximity Ligation Assay (Cell)
      • Representative image of Proximity Ligation Assay of protein-protein interactions between AKT1 and BAD. A-549 cells were stained with anti-AKT1 rabbit purified polyclonal antibody 1:100 and anti-BAD mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
      • In situ Proximity Ligation Assay (Cell)
      • <em>In situ</em> Proximity Ligation Assay (Cell)
      • Representative image of Proximity Ligation Assay of protein-protein interactions between AKT1 and BAD. HT-29 cells were stained with anti-AKT1 rabbit purified polyclonal antibody 1:100 and anti-BAD mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
      • Application Image
      • In situ Proximity Ligation Assay (Cell)
      • <em>In situ</em> Proximity Ligation Assay (Cell)
      • enlarge
      • In situ Proximity Ligation Assay (Cell)
      • <em>In situ</em> Proximity Ligation Assay (Cell)
      • enlarge
      • In situ Proximity Ligation Assay (Cell)
      • <em>In situ</em> Proximity Ligation Assay (Cell)
      • enlarge
      • AKT1
      • BAD
      • Gene Information
      • Entrez GeneID:
      • 207
      • Gene Name:
      • AKT1
      • Gene Alias:
      • AKT,MGC99656,PKB,PKB-ALPHA,PRKBA,RAC,RAC-ALPHA
      • Gene Description:
      • v-akt murine thymoma viral oncogene homolog 1
      • Omim ID:
      • 164730, 181500
      • Gene Ontology:
      • Hyperlink
      • Gene Summary:
      • The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
      • Other Designations:
      • RAC-alpha serine/threonine-protein kinase,murine thymoma viral (v-akt) oncogene homolog-1,protein kinase B,rac protein kinase alpha
      • Gene Information
      • Entrez GeneID:
      • 572
      • Gene Name:
      • BAD
      • Gene Alias:
      • BBC2,BCL2L8
      • Gene Description:
      • BCL2-associated agonist of cell death
      • Omim ID:
      • 603167
      • Gene Ontology:
      • Hyperlink
      • Gene Summary:
      • The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq
      • Other Designations:
      • BCL-X/BCL-2 binding protein,BCL2-antagonist of cell death protein,BCL2-binding component 6,BCL2-binding protein
      • Interactome
      • Interactome
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