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Millipore/DAB500 | IHC Select? HRP/DAB, 500 Tests/DAB500/1 kit500 tests
  • Millipore/DAB500 | IHC Select? HRP/DAB, 500 Tests/DAB500/1 kit500 tests

Millipore/DAB500 | IHC Select? HRP/DAB, 500 Tests/DAB500/1 kit500 tests

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貨號(hào): DAB500
品牌: Millipore
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    • Description
      CatalogueNumberDAB500
      ReplacesDAB250
      BrandFamilyChemicon®
      TradeName
      • IHCSelect
      • Chemicon
      DescriptionIHCSelect?HRP/DAB,500Tests
      OverviewSummaryandExplanation:

      Immunohistochemicaltechniqueshavebeeninuseasadjunctivemethodsfortherecognitionofcellsintissuesectionsforatleast50years.Thefirstmethodreporteduseoffluorescentlabelsin19421.In1974,reportsofusingenzymaticlabels,suchashorserADIshperoxidase(HRP)inroutineH&Eparaffinsectionsappeared2,3.Themethodshavesincebecomethe"standardofcare"insurgicalpathologywhenclassicmethodsalonefailtoyieldadefinitivediagnosis4-9.

      Earlytechniqueswerebasedontheperoxidase-anti-peroxidase(PAP)reaction28,whileimprovementsexploitedthestrongaffinityofavidinforbiotin10.ThistechniqueusesHRP-labeledstreptavidin.Thesecondaryantibodyisconjugatedwithbiotinandthestreptavidin-HRPcomplexreactswiththebiotinonthesecondaryantibodies.Theresultingbiotin-avidin-HRPcomplexcanreactwiththeprimaryantibodyboundtothespecificepitopeofthetargetantigen.TheHRPenzymesofthestreptavidincomplexthencatalyzethesubstrate/chromogenreactiontoformacoloredreactionproduct(browntoblackwhenusingDABasthechromogen)attheantigensite.ABIOLOGicalstainisthenusedtovisualizethewholetissuesection.
      ProductInformation
      Components
      • *20773/50mL-BlockingReagent:normalgoatseruminphosphatebufferedsaline(PBS)containingcarrierprotein
      • *20775/50mL-SecondaryAntibodies:biotinylatedgoatanti-mouseIgGandgoatanti-rabbitIgGinPBS,containingcarrierprotein
      • *20774/50mL-StreptavidinHRP:DilutedinTrisBufferedSaline
      • *71897/4mL-DABChromogenA:3,3"DiaminobenzidinedilutedinTBS
      • *71898/2x50mL-DABChromogenB:hydrogenperoxidedilutedinTBS,containingTween?20
      • *20785/50mL-Hematoxylin:Mayer"shematoxylincounterstain
      • *20784/2x500mL-20XRinseBuffer:TBS
      DetectionmethodChromogenic
      StorageandShippingInformation
      StorageConditionsStABIlityandStorage:

      Whenstoredat2-8°C,theIHCSelect?ImmunoperoxidaseSecondaryDetectionSystemisstableuptotheexpirationdateprintedonthekitlabel.Donotfreezeorexposetoelevatedtemperatures.Discardanyremainingreagentsaftertheexpirationdate.Donotstorekitcomponentsorperformstaininginstronglight,suchasdirectsunlight.

      Precautions:

      ·Pooling,substitutionoralterationofanyreagentmaycauseerroneousresults.

      *Donotmixreagentsfromdifferentlots.

      *Donotallowtheslidestodryatanytimeduringthestainingprocedure.

      *Handleallspecimensandmaterialscomingincontactwiththemaspotentiallyinfectiousmaterials.Disinfectwith0.05%sodiumhypochlorite.

      *DonotmouthPipettereagents.Ifingested,seekprofessionalassistanceorcontactaPoisonControlCenterimmediately.

      ·Diaminobenzidine(DAB)maybecarcinogenic.Thissolutionmaycauseirritationuponskincontact.Avoidinhalation,ingestion,orskincontactandweargloveswhenhandlingDAB.Ifskincontactoccurs,flushaffectedareawithwater.Spillsmaybe"neutralized"withbleach25,26.Disposeofaccordingtolocalregulations.

      ·Inhalationoringestionofxyleneorthehighlyallergenicfixativeformaldehydeisharmful.Thesematerialsshouldbehandledwithgloves.Prepareinafumehood.Ifskinoreyecontactoccurs,washthoroughlywithcopiousamountsofwater.

      ·Weardisposablegloveswhenhandlinganyhumanmaterialandwhileperformingthestainingprocedure.MaterialsRequired:
      ·PrimaryrabbitormouseIgGantibodies(suchasCHEMICONIHCSelect?PredilutedPrimaryAntibodies)
      ·Positiveandnegativetissuecontrolslides
      ·Positivelychargedmicroscopeslides
      ·Glasscoverslips(24x40mm,No.1)
      ·Distilledordeionizedwater
      ·Tween?20
      ·Permanentmountingmedium,Eukitt?AmericanHistologyorequivalent
      ·3%laboratorygradehydrogenperoxide
      ·10.0%neutralbufferedformalin15
      ·Xylene,ACSgrade
      ·Ethanol,absolute(100%)
      ·Latex,rubberor100%nitrilegloves(xyleneresistant)
      ·Timer
      ·Fumehood
      ·LightmicroscopeequippedwithPlanAchromatorequivalentlenses
      ·60°Cdryingoven
      Applications
      KeyApplications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry(Paraffin)
      ApplicationNotesTheIHCSelect?ImmunoperoxidaseSecondaryDetectionSystemisintendedforusewiththeCHEMICONIHCSelect?PredilutedPrimaryAntibodyreagentswhichcontainrabbitandmouseIgGforthequalitativeidentificationofantigensbylightmicroscopyinparaffin-embeddedtissues.TheIHCSelect?ImmunoperoxidaseSecondaryDetectionSystemmayalsobeusedwithotherrabbitormouseIgGprimaryantibodies.

      GeneralPurposeReagents
      BiologicalInformation
      SpeciesReactivity
      • Mouse
      • Rabbit
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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