Histone H4 (Acetyl-Lys5) Antibody detects endogenous levels of total Histone H4 protein only when acetylated at Lys5.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using corresponding non-acetylated peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunofluorescence analysis of HeLa cells, using Histone H4 (Acetyl-Lys5) Antibody. The picture on the right is blocked with the synthesized peptide.
Image 2
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H4 (Acetyl-Lys5) Antibody. The picture on the right is blocked with the synthesized peptide.
Image 3
Western blot analysis of lysates from COS7 cells, treated with TSA 400nM 24h, using Histone H4 (Acetyl-Lys5) Antibody. The lane on the right is blocked with the synthesized peptide.