Mix-n-Stain? CF543 Antibody Labeling Kit is an orange fluorescent dye with absorption and emission maxima at 541 and 560 nm, respectively. Unlike traditional rhodamine dyes, CF?543 is not only bright and photostable but also highly water-soluble. As a result, CF?543 antibody conjugates retain excellent solubility and yield the brightest fluorescence among antibody conjugates prepared from spectrally similar dyes.
Mix-n-Stain? antibody labeling kits are a revolutionary antibody labeling technology that allows you to label your primary antibody with biotin or one of our superior CF? dyes in 30 minutes without the need for a purification step. The labeling procedure tolerates many common buffer components and antibody stabilizers. CF?dyes are highly water soluble, small organic dyes designed for labeling proteins and nucleic acids. Many of our CF? dyes are brighter and more photostable than competing dyes. Click the link for a complete list of more than 20 GoldBio CF? antibody labeling kits.
Technical SummaryAbs/Em Maxima: 541/560 nmExcitation laser line: 532 nm, 543 nm or 546 nmExtinction Coefficient: 100,000MW: ~870 g/mol | | Kit ComponentsDyeMix-n-Stain? 10X reaction bufferMix-n-Stain? antibody storage bufferUltrafiltration vial |
Storage/Handling: Store the kit at -20°C.
Product may be shipped on room temperature without reducing performance. Please place at the recommended storage conditions upon receipt of the product.