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Millipore/CBL407 | Anti-PCNA Antibody, clone PC10/CBL407/100 µg
  • Millipore/CBL407 | Anti-PCNA Antibody, clone PC10/CBL407/100 µg

Millipore/CBL407 | Anti-PCNA Antibody, clone PC10/CBL407/100 µg

價(jià)格: ￥3204.00 市場(chǎng)價(jià): 5340.00
貨號(hào): CBL407
品牌: Millipore
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    • Description
      CatalogueNumberCBL407
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-PCNAAntibody,clonePC10
      AlternateNames
      • ProliferatingCellNuclearAntigen
      • DNAPolymerasedeltaProcessivityFactor
      BackgroundInformationProliferatingcellnuclearantigen(PCNA)isa36kDamoleculethatishighlyconservedbetweenspecies.PCNAwasfirstidentifiedastheantigenforasubpopulationofautoantibodiesinpatientswithsystemiclupuserythematosus(Miyachi,1978;Takasaki,1984;Ogata,1987).IthassincebeendeterminedthatPCNAservesasaco-factorforDNApolymerasedeltainS-phaseaswellasduringDNAsynthesisassociatedwithmechanismsinvolvedinDNAdamagerepair(Tan,1987;Bravo,1987).ThetemporalspecificityofPCNAexpressionmakesitanidealMarkerforcellproliferation.PCNAbeginstoaccumulateduringtheG1phaseofthecellcycle,ismostabundantduringtheSphase,anddeclinesduringtheG2/Mphase(Kurki,1988).Sincethehalf-lifeofPCNAexceeds20hours,itmaybepossIBLetodetecttheproteininnon-cyclingcells.
      ProductInformation
      FormatPurified
      Control
      • POSITIVECONTROL:Tonsilorreactivelymphnode.
      PresentationThemonoclonalispresentedataconcentrationof100μg/1mLinphosphatebufferedsalinecontaining10mMsodiumazideand1mg/mlbovineserumalbumin.Werecommendthateachlaboratorydetermineanoptimumworkingtitreforuseinitsparticularapplication.
      StorageandShippingInformation
      StorageConditionsForusewithin1monthofpurchasestoreat+4°C,forlongtermstoragealiquotantibodyintosmallvolumesandstoreat-20°C.Avoidrepeatedfreeze/thawing.
      Applications
      ApplicationAnti-PCNAAntibody,clonePC10isamousemonoclonalantibodyfordetectionofPCNAalsoknownasProliferatingCellNuclearAntigen,DNAPolymerasedeltaProcessivityFactor&hasbeenvalidatedinELISA,FC,IP,WB,IHC(P).
      KeyApplications
      • ELISA
      • FlowCytometry
      • Immunoprecipitation
      • WesternBlotting
      • Immunohistochemistry(Paraffin)
      ApplicationNotesWesternblot:1:250-1:1000.Nuclearextractsarepreferredoverwholecellproteinextracts.

      Immunohistochemistry:1:20-1:200.Recommendedforparaffinembeddedtissuesectionsonly.Maybeusedonmaterialfixedinawiderangeoffixativesincludingformalin(bufferedandunbuffered),methacarnandBouin"sreagent.ThetimeoffixationcanmarkedlyaffecttheintensityofPCNAimmunoreactivity.Stainingisreduced(andmaybeabolished)ifsectionsarebakedontoglassslides.Air-dryingovernightonpoly-L-lysinecoatedslidesisrecommended.60minuteincubationat25°CwithstandardABCtechniqueisrecommended.

      Immunoprecipitation

      IndirectFlowCytometry

      Optimalworkingdilutionsmustbedeterminedbytheenduser.
      BIOLOGicalInformation
      ImmunogenRatPCNAmadeintheproteinAvectorpR1T2T.
      ClonePC10
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityClonePC10recognizesPCNAfromallvertebrateandinsectspeciestestedandhasbeenusedtoidentifytransformedcells(Kurki,1988),proliferatingcellsinsolidtumors(Smetana,1983),andblastcellsinleukemiapatients(Takasaki,1984).ImmunohistochemistrywithclonePC10hasbeenusedtostudytheexpressionofPCNAinparaffinsectionsofnormaltissuesandlymphoidneoplasms(Hall,1990).Inproliferatingcells,PCNAstainingpatternispredominantlynuclear.Bywesternblot,theantibodydetectsapolypeptidemigratingat36kDawithanisoelectricpointof4.8.
      IsotypeIgG2a
      SpeciesReactivity
      • Invertebrates
      • Vertebrates
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryTheproteinencodedbythisgeneisfoundinthenucleusandisacofactorofDNApolymerasedelta.TheencodedproteinactsasahomotrimerandhelpsincreasetheprocessivityofleADIngstrandsynthesisduringDNAreplication.InresponsetoDNAdamage,thisproteinisubiquitinatedandisinvolvedintheRAD6-dependentDNArepairpathway.Twotranscriptvariantsencodingthesameproteinhavebeenfoundforthisgene.Pseudogenesofthisgenehavebeendescribedonchromosome4andontheXchromosome.
      GeneSymbol
      • PCNA
      • Cyclin
      • cyclin
      • MGC8367
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P12004#ThisproteinisanauxiliaryproteinofDNApolymerasedeltaandisinvolvedinthecontrolofeukaryoticDNAreplicationbyincreasingthepolymerase"sprocessibilityduringelongationoftheleadingstrand.
      SIZE:261aminoacids;28769Da
      SUBUNIT:Homotrimer.InteractswithKCTD10(Bysimilarity).Formsacomplexwithactivator1heteropentamerinthepresenceofATP.InteractswithPOLH,POLK,DNMT1,ERCC5/XPG,FEN1,CDC6,APEX2andPOLDIP2.InteractswithEXO1andSHPRH.
      SUBCELLULARLOCATION:Nucleus.
      PTM:Uponmethylmethanesulfonate-inducedDNAdamage,mono-ubiquitinatedbytheUBE2B-RAD18complexonLys-164.Thisinducesnon-canonicalpoly-ubiquitinationonLys-164through"Lys-63"linkageofubiquitinmoietiesbytheE2complexUBE2N-UBE2V2andtheE3ligaseSHPRH,whihisrequiredforDNArepair.
      DISEASE:SwissProt:P12004#Antibodiesarepresentinserafrompatientswithsystemiclupuserythematosus.
      SIMILARITY:SwissProt:P12004##BelongstothePCNAfamily.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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