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Millipore/CBL1512 | Anti-Integrin αM [CD11b] Antibody, clone OX-42/CBL1512/500 µg
  • Millipore/CBL1512 | Anti-Integrin αM [CD11b] Antibody, clone OX-42/CBL1512/500 µg

Millipore/CBL1512 | Anti-Integrin αM [CD11b] Antibody, clone OX-42/CBL1512/500 µg

價(jià)格: ¥4068.00 市場(chǎng)價(jià): 6780.00

貨號(hào): CBL1512
品牌: Millipore
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    • Description
      CatalogueNumberCBL1512
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-IntegrinαM[CD11b]Antibody,cloneOX-42
      AlternateNames
      • CD11b
      BackgroundInformationIntegrinalphaM/beta2isimplicatedinvariousadhesiveinteractionsofmonocytes,macrophagesandgranulocytesaswellasinmediatingtheuptakeofcomplement-coatedparticles.ItisidenticalwithCR3,thereceptorfortheiC3bfragmentofthethirdcomplementcomponent.ItprobablyrecognizestheR-G-DpeptideinC3b.IntegrinalphaM/beta2isalsoareceptorforfibrinogen,factorXandICAM1.ItrecognizesP1andP2peptidesoffibrinogengammachain.
      ProductInformation
      FormatPurified
      Control
      • Braintissue
      PresentationThemonoclonalispresentedinphosphatebufferedsalinecontaining10mMsodiumazide.
      StorageandShippingInformation
      StorageConditionsForusewithin1monthofpurchasestoreat+4°C,forlongtermstoragealiquotantibodyintosmallvolumesandstoreat-20°C.
      Applications
      ApplicationDetectIntegrinαM[CD11b]usingthisAnti-IntegrinαMCD11bAntibody,cloneOX-42validatedforuseinFC,IH,IH(P)&IP.
      KeyApplications
      • FlowCytometry
      • Immunohistochemistry
      • Immunohistochemistry(Paraffin)
      • Immunoprecipitation
      ApplicationNotesFlowcytometry

      Immunohistochemicalstudiesofratfreshfrozentissuesectionsandparaffin-embeddedtissuesectionsfollowingeitherperiodate-lysine-paraformaldehyde(PLP)fixation,oracetone.WorksonverylightlyPFAfixed,frozentissues.(perfusiononly4%PFA10-15"nopost-fix).

      Immunoprecipitation:userabbitanti-mouseoranti-mouseIgGbeadforcaptureonly.TheuseofproteinAorproteinGisnotrecommended.

      Optimalworkingdilutionsmustbedeterminedbytheenduser.
      BIOLOGicalInformation
      ImmunogenRatperitonealmacrophages
      CloneOX-42
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityRecognizesmostmacrophages(includingresidentperitonealandactivatedmacrophages),kupffercells,butonlyabout35%ofalveolarmacrophages.Theantibodyalsolabelsdendriticcellsextensively,granulocytesandcellswiththemorphologyofmicrogliainbrain.Theantibodyprecipitatesthreepolypeptidesof150,103and95KDa.TheantibodyinhibitscomplementmediatedrosettesandisprobablytheratequivalentofthehumanreceptorforIC3bcalledCR3.
      FUSIONPARTNER:NSOmyelomacellline.
      IsotypeIgG2a
      SpeciesReactivity
      • Rat
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodestheintegrinalphaMchain.Integrinsareheterodimericintegralmembraneproteinscomposedofanalphachainandabetachain.ThisI-domaincontainingalphaintegrincombineswiththebeta2chain(ITGB2)toformaleukocyte-specificintegrinreferredtoasmacrophagereceptor1("Mac-1"),orinactivated-C3b(iC3b)receptor3("CR3").ThealphaMbeta2integrinisimportantintheadherenceofneutrophilsandmonocytestostimulatedendothelium,andalsointhephagocytosisofcomplementcoatedparticles.SomeintegrinalphaMproteinscontainaninsertedaminoacid(Q,betweenAApositions499and500)resultingfroma3bpshiftinaspliceacceptorthatinsertsthecodonCAG;receptorswithorwithouttheextraaminoacidarefunctionallyindistinguishable
      GeneSymbol
      • ITGAM
      • MO1A
      • CD11b
      • MAC1A
      • CD11B
      • MAC-1
      • CR3A
      • MGC117044
      PurificationMethodProteinAPurfied
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P11215#Integrinalpha-M/beta-2isimplicatedinvariousadhesiveinteractionsofmonocytes,macrophagesandgranulocytesaswellasinmediatingtheuptakeofcomplement-coatedparticles.ItisidenticalwithCR-3,thereceptorfortheiC3bfragmentofthethirdcomplementcomponent.ItprobablyrecognizestheR-G-DpeptideinC3b.Integrinalpha-M/beta-2isalsoareceptorforfibrinogen,factorXandICAM1.ItrecognizesP1andP2peptidesoffibrinogengammachain.
      SIZE:1152aminoacids;127179Da
      SUBUNIT:Heterodimerofanalphaandabetasubunit.Alpha-Massociateswithbeta-2.
      SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein.
      TISSUESPECIFICITY:Predominantlyexpressedinmonocytesandgranulocytes.
      DOMAIN:SwissProt:P11215TheintegrinI-domain(insert)isaVWFAdomain.IntegrinswithI-domainsdonotundergoproteasecleavage.
      SIMILARITY:Belongstotheintegrinalphachainfamily.&Contains7FG-GAPrepeats.&Contains1VWFAdomain.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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